Analytical

分析型

• 批准号: 7631370
• 负责人: Julian P Whitelegge
• 金额: $ 18.95万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7631370
• 关键词: Amyotrophic Lateral Sclerosis; Base Sequence; Biological Assay; Chemicals; Chemistry; Collaborations; Copper; Data; Detection; Heterogeneity; Human; In Vitro; Lasers; Mass Spectrum Analysis; Measurement; Metals; Modification; Molecular; Molecular Sieve Chromatography; Molecular Weight; Mus; Mutant Strains Mice; Neurodegenerative Disorders; Open Reading Frames; Plasma; Post-Translational Protein Processing; Preparation; Principal Investigator; Process; Protein Analysis; Proteins; Proteomics; Range; Reactive Oxygen Species; Research Personnel; Role; Services; Shotguns; Site; Solutions; Sorting - Cell Movement; Source; Spinal Cord; Stress; Testing; Transgenic Organisms; Translations; Variant; Vision; Zinc; base; genome sequencing; in vivo; insight; interest; light scattering; mouse model; mutant; nitration; oxidation; programs; protein aggregate; protein aminoacid sequence; size; tandem mass spectrometry

Principal Investigator/Program Director (Last, first, middle): Valentine, Joan S. - Core A WhiteleQQe. Julian P. DESCRIPTION: The role of analytical Core A is to provide a comprehensive range of services for the physical, chemical and proteomic characterization of SOD-containing and other aggregates derived from both in vivo transgenic ALS mouse models and in vitro from SOD1 mutants treated with potential pro-oxidants. Importantly, our analyses are based on a discovery-based approach that does not rely upon preconceptions as to what we may be lookingfor. Specific Aim 1. Proteomic analysis of aggregates Specific Aim 2. Metal analysis of aggregates and SOD1 protein preparations. Specific Aim 3. Aggregate heterogeneity and molecular weight. Specific Aim 4. Protein mass profiling and covalent modification sites.

首席调查员/计划总监（最后，第一，中间）：琼·S·瓦伦丁 - 核心A Whiteleqqe。朱利安·P· 描述： 分析核心A的作用是为物理， 含SOD和其他骨料的化学和蛋白质组学表征 来自体内转基因ALS小鼠模型和从SOD1突变体的体外 潜在的促氧化剂。重要的是，我们的分析基于基于发现的方法 这并不依赖于我们可能正在寻找的成就。 特定目的1。聚集体的蛋白质组学分析 具体目标2。聚集体和SOD1蛋白制剂的金属分析。 特定目标3。骨料异质性和分子量。 特定目标4。蛋白质质量分析和共价修饰位点。