Graphene Based Nanocomposites for Bone Tissue Engineering

用于骨组织工程的石墨烯基纳米复合材料

基本信息

批准号：
9925837
负责人：
ADEREMI R OKI
金额：
$ 11.18万
依托单位：
PRAIRIE VIEW AGRI & MECH UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-01 至 2021-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9925837
关键词：
Ablation Amines Area Behavior Biological Biomedical Research Body Fluids Bone Tissue Breast Caliber Cancer Patient Cations Cell Death Cells Chemistry Collaborations Copper Development Electromagnetic Energy Encapsulated Exposure to Folic Acid Frequencies Goals Gold Growth Head and Neck Cancer Heparin Human In Vitro Induced Hyperthermia Iodine Lasers Length Ligands Light Liquid substance Lung Manuscripts Mediating Metabolism Methods Monoclonal Antibodies Nanostructures Operative Surgical Procedures Ovarian Oxidation-Reduction Penetration Photobleaching Photosensitization Photosensitizing Agents Physiological Polysaccharides Procedures Process Property Publications Quantum Dots Radiation therapy Reaction Reporting Reproducibility Research Semiconductors Solubility Source Structure Students Sulfhydryl Compounds Sulfides Surface Temperature Thermal Ablation Therapy Tissue Engineering Tissues Uterus Vitamin B Complex Water Work Yang absorption alternative treatment analog base cancer cell cancer site cell killing chemotherapy cost density dodecane effective therapy experimental study folate-binding protein graphene improved interest ion mobility minimally invasive nanocomposite nanocrystal nanodisk nanoparticle nanorod nanosized nanosystems neoplastic cell organic base outreach overexpression plasmonics receptor response stoichiometry success targeted agent targeted delivery tumor uptake

项目摘要

Project Summary The proposal deals with the development of simple synthetic procedure for fabricating cation deficient copper (1) sulfides, Cu2-xS nanorods of varying aspect ratio and the encapsulation in folic acid conjugated heparin to form a bio-conjugate, HF-CuS . This bio-conjugate will have the capabilities to “seek and attach itself to tumor cells due to the overexpression of folate receptors in such cells. The transport ability of such matrix in physiological fluid will also be enhanced due to the solubility of the heparin, it ability to coat the surface of copper sulfide as a non-cytotoxic, biodegradable polysaccharide. The Cu2-xS should be able to thermally ablate cancer cells when exposed to near infra-red laser light (NIR) of desired wavelength. The NIR laser is less absorbed by biological tissues with typical tissue penetration depth approaching several centimeters for 980 nm light. The work will be accomplished in three steps: 1. The fabrication of Cu2-xS nanorods with various aspect ratios, will be accomplished using a combination of 1-DDT (1 dodecane thiol) and t-DDT (tert-dodecane thiol) as the source of sulfide and also the structure directing ligands through manipulation of growth and nucleation temperature; 2. While it is well known that the photo thermal conversion efficiency in Cu2-XS nanoparticles are usually less effective when compared to the Gold nanosystem, however, the plasmon properties in Cu2-xS are due to carrier holes which can be easily varied through control of the stoichiometry. This will be accomplished by taking advantage of Cu+ ion mobility in Chalcogenides, and its surface redox reactions with soluble amines to optimize the plasmonic properties and finally, the encapsulation of the Cu2-XS nanorods with folic acid conjugated heparin will enhance the matrix mobility and targeting ability for tumor cells and photo thermal conversion efficiency. Folic acid , the soluble form of vitamin B, targets tumors in a similar manner to monoclonal antibodies, as foliate receptors (FRs) are overexpressed in more than 40% of human tumors and are generally absent in healthy cells . The heparin will enhance the matrix transport properties in biological fluid or simulated body fluid through coating on the surface of the copper sulfide. If the proposed research is successfully carried out, a new matrix will emerge for targeted delivery to cancer cells and ablation of cancer cells more so in areas where surgical procedure or radiation therapy has limited success.

项目概要该提案涉及开发用于制造阳离子的简单合成程序缺铜 (1) 硫化物、不同纵横比的 Cu2-xS 纳米棒以及叶酸中的封装酸结合肝素形成生物结合物 HF-CuS，该生物结合物将具有：由于叶酸受体在此类细胞中的过度表达，因此具有“寻找并附着在肿瘤细胞上的能力” 由于细胞的存在，这种基质在生理液中的转运能力也会增强。肝素的溶解度，它能够以非细胞毒性、可生物降解的形式覆盖硫化铜的表面当暴露于附近时，Cu2-xS 应该能够热消融癌细胞。所需波长的红外激光 (NIR) NIR 激光被生物组织吸收较少。 980 nm 光的典型组织穿透深度接近几厘米。分三步完成： 1. 制备不同长宽比的 Cu2-xS 纳米棒，将使用 1-DDT（1 十二烷硫醇）和 t-DDT（叔十二烷硫醇）的组合来完成硫化物的来源以及通过操纵生长和引导配体的结构成核温度；2.众所周知，Cu2-XS的光热转换效率与金纳米系统相比，纳米粒子通常效果较差，但是，等离子体激元 Cu2-xS 中的特性是由于载流子孔，可以通过控制这将通过利用硫属化物中的 Cu+ 离子淌度来实现，及其与可溶性胺的表面氧化还原反应，以优化等离子体特性，最后，用叶酸结合肝素封装 Cu2-XS 纳米棒将增强基质肿瘤细胞的移动性和靶向能力以及光热转换效率。可溶形式的维生素 B，以与单克隆抗体类似的方式靶向肿瘤，如叶酸受体（FR）在超过 40% 的人类肿瘤中过度表达，而在健康细胞中的肝素将增强生物液体或模拟物中的基质运输特性。体液通过涂覆在硫化铜的表面如果建议的研究是。如果成功实施，将出现一种新的基质，用于靶向递送至癌细胞并消融癌细胞更多地出现在外科手术或放射治疗效果有限的区域。