A NEW MODEL FOR FEAR LEARNING IN TROUT

鳟鱼恐惧学习的新模型

基本信息

批准号：
7627585
负责人：
CLIFF H SUMMERS
金额：
$ 0.48万
依托单位：
UNIVERSITY OF SOUTH DAKOTA
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-06-01 至 2008-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7627585
关键词：
Adopted Aggressive behavior Auditory Behavioral Brain Brain region Computer Retrieval of Information on Scientific Projects Database Condition Conditioned Stimulus Elements Environment Fishes Fright Funding Grant Hydrocortisone Institution Learning Lizards Microdissection Modeling Neuropeptides Oncorhynchus mykiss Plasma Polymerase Chain Reaction Receptor Gene Research Research Personnel Resources Rodent Model Role Route Salmo trutta Source Stimulus Stress and Coping Testing Time United States National Institutes of Health Work conditioned fear day design morris water maze response size social social stress somatosensory stressor

Adopted Aggressive behavior Auditory Behavioral Brain Brain region Computer Retrieval of Information on Scientific Projects Database Condition Conditioned Stimulus Elements Environment Fishes Fright Funding Grant Hydrocortisone Institution Learning Lizards Microdissection Modeling Neuropeptides Oncorhynchus mykiss Plasma Polymerase Chain Reaction Receptor Gene Research Research Personnel Resources Rodent Model Role Route Salmo trutta Source Stimulus Stress and Coping Testing Time United States National Institutes of Health Work conditioned fear day design morris water maze response size social social stress somatosensory stressor

展开

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to develop a new model for fear conditioning using rainbow trout. This model combines elements discovered during our recent work on social stress in lizards and rainbow trout with well defined rodent models for spatial learning (Morris water maze) and fear conditioning. Social aggression is the most potent stressor known, which we utilize in the form of a march larger intruder fish as an unconditioned stimulus (US). This social aggression US is paired with an auditory/somatosensory conditioned stimulus (CS) in context of an escape route (hole) available only to the smaller resident test fish (due to size). Fish either learn to escape from social aggression or remain in the tank to be attacked and subordinated. After seven days of CS-US paairing, on the eighth day the CS is presented alone (without the larger fish), and fish respond either by escaping or by a physiologically conditioned response, elevated plasma cortisol. The model is designed to be used for testing spatial learning in a highly fearful situation, and producing fear conditioning using contextually and ecologically relevant stimuli and behavioral responses. We hypothesize that the neuropeptide CRF regulates which stress coping strategies are adopted by trout during learning in a socially aggressive environment. The aims of this proposal are to: 1. Test and refine the experimental parameters of the model 2. Prove that we can obtain repeatable results 3. Begin to distinguish the role of the CRF in stress coping strategies during fear learning 4. Compare expression of CRF and CRF receptor genes in specific brain regions of trout that do and don't learn to escape using brain punch microdissection and real-time PCR

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。我们建议开发一种新的模型，用于使用彩虹鳟鱼恐惧调理。该模型结合了我们最近在蜥蜴和彩虹鳟鱼中的社会压力工作中发现的元素，以及定义明确的啮齿动物模型用于空间学习（Morris Water Maze）和恐惧调节。社会侵略是已知的最有效的压力源，我们以较大的入侵者鱼作为无条件的刺激（美国）的形式使用。这种社交侵略性美国与听觉/体感条件刺激（CS）配对，在逃生路线（孔）的背景下仅可用于较小的居民测试鱼（由于尺寸）。鱼要么学会逃脱社交侵略，要么留在坦克中被攻击和服从。在CS-US吞噬7天后，在第八天，CS单独出现（没有较大的鱼），鱼通过逃脱或生理条件的反应来反应，血浆皮质醇升高。该模型旨在在高度恐惧的情况下用于测试空间学习，并使用上下文和生态相关的刺激和行为反应产生恐惧调节。我们假设神经肽CRF调节在社会侵略性环境中学习过程中鳟鱼采用的压力应对策略。该提案的目的是： 1。测试和完善模型的实验参数 2。证明我们可以获得可重复的结果 3。开始区分CRF在恐惧学习过程中的压力应对策略中的作用 4。比较在特定的鳟鱼大脑区域中CRF和CRF受体基因的表达，这些区域可以使用脑拳显微解剖和实时PCR逃脱逃脱