FAMILY STUDY

家庭学习

基本信息

批准号：
7607810
负责人：
CHARLES Nohuoma ROTIMI
金额：
$ 52.02万
依托单位：
HOWARD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-03-01 至 2008-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7607810
关键词：
ADRB2 gene Adrenergic Receptor African American Age Aldosterone Angiotensinogen Appointment Area Blood Pressure Blood specimen Body Composition Body fat C-reactive protein Clinic Communities Computer Retrieval of Information on Scientific Projects Database Consent DNA Data Diabetes Mellitus District of Columbia Edetic Acid Education Endothelin-1 Enrollment Epithelial Family Family Study Family history of Family member Fasting Fatty acid glycerol esters Funding Genes Glucose Goals Grant Gray unit of radiation dose Height Hip region structure Hour Household Hypertension Income Informed Consent Institution Insulin Measurement Measures Medical History Methods Oxides Participant Peptides Peptidyl-Dipeptidase A Persons Physiological Plasma Population Receptor, Angiotensin, Type 1 Renin-Angiotensin-Aldosterone System Research Research Personnel Resources Schedule Serum Smoking Sodium Channel Source Spottings Tube United States National Institutes of Health Urine Weight Writing base cohort cysteine rich protein drinking electric impedance epithelial Na+ channel family structure follow-up genetic epidemiology metropolitan proband size

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The study is examining the genetic epidemiology of hypertension in a cohort of African Americans in the Washington, DC metropolitian area. The specific goals are to i) Enroll and examine a randomly acertained population-based cohort of 350 AA families with a minimum of 5 persons per family ii) Collect extensive demographic data (eg. adddress for anticipated follow-up, income education, smoking, drinking and household size) and family medical history on all participants , iii) Perform extensive phenotypic characterization, including: a) anthropometric and related variables: height, weight, waist and hip circumference, body composition variables, including fat mass (FM), fat free mass (FFM), and percent body fat (PBF) using Bioelectric Impedance Analyzer (BIA) in all participants b) BP and related physiologic intermediates including components of the renin-angiotensin aldosterone system (RAAS): angiotensinogen (AGT), angiotensin converting enzyme (ACE) and aldosterone. Measure other BP related intermediate pheotypes including endothelin 1 (ET1) and C-reactive protein(CRP), c) diabetes related variables: glucose, c-peptide and insulin in all participants. iv) Evaluate the association and linkage between BP, HTN and genes of the RAAS (ACE, AGT and angiotensin II receptor type 1- AT2R1) and other selected condidate genes including epithelial sodium channel beta subunit (ENaC), endothelial nitic oxide synthases (eNOSs\, CRP, ET1, and beta2-adrenergic receptor (ADRB2). Probands satisfying the age requirement (i.e., 30-60 years) and family structure criteria are identified from randomly selected, predominantly African American communities from the DC metropolitan area, after which the proband and consenting family members are scheduled for a clinic appointment in the GCRC. Participants are requested to fast for a minimum of eight hours on the night preceding the clinic appointment. At the GCRC, written informed consent is obtained following which demographic data and a medical history are obtained. Anthropometric, blood pressure and body composition measurements are done using standardized methods. Fasting blood samples are obtained from each participant comprising two 10ml EDTA tubes for plasma and buffy coat (source of DNA), one 15ml red top for serum and a 5 ml gray top for fasting glucose measurement. Spot urine are also obtained from all participants.

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目和研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。该研究正在华盛顿特区大都市区的一组非裔美国人中研究高血压的遗传流行病学。具体目标是 i) 登记并检查随机确定的 350 个 AA 家庭队列，每个家庭至少有 5 人 ii) 收集广泛的人口统计数据（例如预期随访的地址、收入教育、吸烟、饮酒和家庭规模）以及所有参与者的家族病史，iii）进行广泛的表型表征，包括：a）人体测量和相关变量：身高、体重、腰围和臀围、身体成分变量，包括脂肪量使用生物电阻抗分析仪 (BIA) 对所有参与者进行 (FM)、去脂体重 (FFM) 和体脂百分比 (PBF) b) 血压和相关生理中间体，包括肾素-血管紧张素醛固酮系统 (RAAS) 的成分：血管紧张素原（ AGT）、血管紧张素转换酶（ACE）和醛固酮。测量所有参与者的其他血压相关中间表型，包括内皮素 1 (ET1) 和 C 反应蛋白 (CRP)，c) 糖尿病相关变量：葡萄糖、C 肽和胰岛素。 iv) 评估 BP、HTN 和 RAAS 基因（ACE、AGT 和血管紧张素 II 受体 1 型 - AT2R1）和其他选定的候选基因（包括上皮钠通道 β 亚基 (ENaC)、内皮一氧化氮合酶 (eNOS)）之间的关联和联系\、CRP、ET1 和 β2-肾上腺素能受体 (ADRB2)。满足年龄要求（即 30-60 岁）和家庭结构标准的先证者是从 DC 大都市区随机选择的、以非裔美国人为主的社区中确定的，之后先证者和同意的家庭成员被安排在 GCRC 进行诊所预约。要求参与者在诊所预约前一天晚上禁食至少八小时。在 GCRC，先获得书面知情同意书，然后再获得人口统计数据和病史。人体测量、血压和身体成分测量均使用标准化方法进行。从每个参与者处获得空腹血样，包括两个用于血浆和血沉棕黄层（DNA 来源）的 10ml EDTA 管、一个用于血清的 15ml 红顶管和一个用于空腹血糖测量的 5ml 灰顶管。还从所有参与者处获得现场尿液。