PRENATAL TOBACCO DEPARTMENT OF PUBLIC HEALTH ((DPH) PROJECT 3)

公共卫生产前烟草部（(DPH) 项目 3）

• 批准号: 7607643
• 负责人: BRUCE J MORRIS
• 金额: $ 0.04万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7607643
• 关键词: Cell Count; Cell surface; Child; Childhood; Computer Retrieval of Information on Scientific Projects Database; Control Groups; Disease; Erythroid; Erythroid Cells; Flow Cytometry; Funding; Future; Grant; Hematopoiesis; Hematopoietic System; Hematopoietic stem cells; Immune system; Incidence; Institution; Lung diseases; Lymphoid; Maintenance; Monoclonal Antibodies; Mus; Myelogenous; Nicotine; Numbers; Pilot Projects; Population; Pregnancy; Prevention; Public Health; Research; Research Personnel; Resources; Role; Smoker; Source; Stem Cell Development; Stem cells; T-Lymphocyte; Tobacco; Umbilical Cord Blood; United States National Institutes of Health; Week; Work; adult stem cell; fetal tobacco exposure; insight; non-smoker; prenatal; prenatal environmental exposure; prenatal influence; progenitor; repaired; response; size; tobacco exposure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The influence of prenatal factors upon stem cell development during the nine months of gestation has the potential to impact lifelong maintenance and repair. A paucity of research has been done on the influence of prenatal environmental exposures upon the quantify of adult stem cell and progenitor subpopulations.1,2 In the hematopoietic system the subpopulations are responsible for establishment and maintenance of the immune system. Studies in mice have shown that nicotine exposure alters both Hematopoietic Stem Cell (HSC) number and hematopoietic progenitor cell function3. Other work has shown an increased incidence of respiratory diseases and a difference in the T cell response in children born to smokers.4,5 This is a pilot project to study the influence of prenatal tobacco exposure on the quantity of hematopoietic stem cells and early progenitor cells in the umbilical cord blood of the offspring of smokers. Prenatal tobacco exposure may alter both the absolute number of these populations as well as the ratio of early progenitor cells to hematopoietic stem cells. Previous research has shown that the expression of early progenitor populations, specifically myeloid/Erythroid and lymphoid progenitors can reflect functional studies on these cells6,7,8,9. It is hoped that studying HSC and progenitor populations will give insight into the impact of tobacco exposure on the role of the stem cell in repair, maintenance, and prevention of childhood disease. Umbilical cord blood will be collected from 31 singleton smokers greater than 32 weeks gestation and a control group of 31 nonsmokers. Using commercially available monoclonal antibodies directed against cell surface markers flow cytometry will be used to quantify the HSC and subpopulations of lymphoid, myeloid, and erythroid cells. This will allow us to analyze the possible influence of prenatal tobacco exposure in early hematopoiesis. In addition, we would like to establish an effect size of the influence of prenatal tobacco exposure on HSC and populations of progenitor necessary to conduct future studies examining statistical significance.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 妊娠九个月期间，产前因素对干细胞发育的影响有可能影响终生的维持和修复。关于产前环境暴露对成体干细胞和祖细胞亚群定量的影响的研究很少。1,2 在造血系统中，亚群负责免疫系统的建立和维持。对小鼠的研究表明，接触尼古丁会改变造血干细胞 (HSC) 数量和造血祖细胞功能3。其他研究表明，吸烟者所生儿童呼吸系统疾病的发病率有所增加，并且 T 细胞反应存在差异。4,5 这是一个试点项目，旨在研究产前烟草暴露对吸烟者后代脐带血中造血干细胞和早期祖细胞数量的影响。 产前接触烟草可能会改变这些群体的绝对数量以及早期祖细胞与造血干细胞的比例。 先前的研究表明，早期祖细胞群，特别是骨髓/红细胞和淋巴祖细胞的表达可以反映这些细胞的功能研究6,7,8,9。希望研究 HSC 和祖细胞群能够深入了解烟草暴露对干细胞在修复、维持和预防儿童疾病中的作用的影响。 将从 31 名妊娠超过 32 周的单身吸烟者和 31 名不吸烟者组成的对照组中采集脐带血。 使用针对细胞表面标志物的市售单克隆抗体，流式细胞术将用于量化 HSC 以及淋巴、骨髓和红细胞的亚群。 这将使我们能够分析产前烟草暴露对早期造血的可能影响。 此外，我们希望确定产前烟草暴露对 HSC 和祖细胞群影响的效应大小，这对于未来进行检验统计显着性的研究是必要的。