PRENATAL TOBACCO DEPARTMENT OF PUBLIC HEALTH ((DPH) PROJECT 3)

公共卫生产前烟草部（(DPH) 项目 3）

• 批准号: 7607643
• 负责人: BRUCE J MORRIS
• 金额: $ 0.04万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7607643
• 关键词: Cell Count; Cell surface; Child; Childhood; Computer Retrieval of Information on Scientific Projects Database; Control Groups; Disease; Erythroid; Erythroid Cells; Flow Cytometry; Funding; Future; Grant; Hematopoiesis; Hematopoietic System; Hematopoietic stem cells; Immune system; Incidence; Institution; Lung diseases; Lymphoid; Maintenance; Monoclonal Antibodies; Mus; Myelogenous; Nicotine; Numbers; Pilot Projects; Population; Pregnancy; Prevention; Public Health; Research; Research Personnel; Resources; Role; Smoker; Source; Stem Cell Development; Stem cells; T-Lymphocyte; Tobacco; Umbilical Cord Blood; United States National Institutes of Health; Week; Work; adult stem cell; fetal tobacco exposure; insight; non-smoker; prenatal; prenatal environmental exposure; prenatal influence; progenitor; repaired; response; size; tobacco exposure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The influence of prenatal factors upon stem cell development during the nine months of gestation has the potential to impact lifelong maintenance and repair. A paucity of research has been done on the influence of prenatal environmental exposures upon the quantify of adult stem cell and progenitor subpopulations.1,2 In the hematopoietic system the subpopulations are responsible for establishment and maintenance of the immune system. Studies in mice have shown that nicotine exposure alters both Hematopoietic Stem Cell (HSC) number and hematopoietic progenitor cell function3. Other work has shown an increased incidence of respiratory diseases and a difference in the T cell response in children born to smokers.4,5 This is a pilot project to study the influence of prenatal tobacco exposure on the quantity of hematopoietic stem cells and early progenitor cells in the umbilical cord blood of the offspring of smokers. Prenatal tobacco exposure may alter both the absolute number of these populations as well as the ratio of early progenitor cells to hematopoietic stem cells. Previous research has shown that the expression of early progenitor populations, specifically myeloid/Erythroid and lymphoid progenitors can reflect functional studies on these cells6,7,8,9. It is hoped that studying HSC and progenitor populations will give insight into the impact of tobacco exposure on the role of the stem cell in repair, maintenance, and prevention of childhood disease. Umbilical cord blood will be collected from 31 singleton smokers greater than 32 weeks gestation and a control group of 31 nonsmokers. Using commercially available monoclonal antibodies directed against cell surface markers flow cytometry will be used to quantify the HSC and subpopulations of lymphoid, myeloid, and erythroid cells. This will allow us to analyze the possible influence of prenatal tobacco exposure in early hematopoiesis. In addition, we would like to establish an effect size of the influence of prenatal tobacco exposure on HSC and populations of progenitor necessary to conduct future studies examining statistical significance.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在妊娠9个月内，产前因素对干细胞发育的影响有可能影响终身维持和修复。对产前环境暴露对量化成年干细胞和祖细胞亚群的影响的研究很少。1,2在造血系统中，亚种群负责建立和维持免疫系统。在小鼠中的研究表明，尼古丁暴露会改变造血干细胞（HSC）数量和造血祖细胞功能3。其他工作表明，吸烟者所生的儿童的呼吸道疾病发生率增加和T细胞反应的差异。4,5 这是一个试点项目，旨在研究产前烟草暴露对吸烟者后代的脐带血中造血干细胞和早期祖细胞数量的影响。 产前烟草暴露可能会改变这些人群的绝对数量，以及早期祖细胞与造血干细胞的比率。 先前的研究表明，早期祖细胞种群，特别是髓样/红细胞和淋巴样祖细胞的表达可以反映对这些细胞的功能研究6,7,8,9。希望研究HSC和祖先种群能够深入了解烟草暴露对干细胞在修复，维持和预防儿童疾病中作用的影响。 将从32周妊娠的31个单胎吸烟者和31个非吸烟者的对照组中收集脐带血。 使用针对细胞表面标志物流式细胞仪的商业可用的单克隆抗体将用于量化淋巴样，髓样和红细胞细胞的HSC和亚群。 这将使我们能够分析产前烟草暴露于早期造血的可能影响。 此外，我们要建立产前烟草暴露对HSC和祖先种群的影响的影响，以进行未来的研究检查统计学意义。