Dissecting the Regulation of Gene Expression during C. elegans Embryogenesis

剖析线虫胚胎发生过程中基因表达的调控

基本信息

批准号：
7534784
负责人：
John Isaac Murray
金额：
$ 8.83万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-12-01 至 2009-11-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): I propose to develop tools, datasets and methods for the genome-wide identification of genes regulated by transcription factors during the embryonic development of Caenorhabditis elegans. During the career development phase, I will use methods we have developed in the Waterston lab to generate an initial map of transcription factor activity with cellular resolution in the C. elegans embryo. In parallel I will develop technology to facilitate the efficient identification of downstream targets of transcription factors in the embryo. Once in my own lab, I will extend these methods to identify and characterize functional targets of many transcription factors on a genomic scale and to explore how transcription factors combine to regulate gene expression. The career development phase will be critical for this effort because of the need for development of methods, and because the transcription factor expression map I propose to produce will provide a critical integrating platform to use when choosing factors for genome-wide analysis and when interpreting the resulting data. Relevance to public health: Knowledge of how transcription factors act in development may provide information about how the same transcription factors function in cancers when inappropriately activated. Furthermore, human orthologs of genes identified in this study as regulated by developmentally important transcription factors may play roles in both normal development and disease.

描述（由申请人提供）：我建议在秀丽隐杆线虫的胚胎发育过程中开发针对转录因子调节的基因鉴定的工具，数据集和方法。在职业发展阶段，我将使用我们在Waterston Lab中开发的方法来生成具有秀丽隐杆线虫胚胎中细胞分辨率的转录因子活性的初始图。同时，我将开发技术，以促进胚胎中转录因子下游靶标有效识别。一旦进入我自己的实验室，我将扩展这些方法，以识别和表征基因组量表许多转录因子的功能靶标，并探索转录因子如何结合以调节基因表达。职业发展阶段对于这项工作至关重要，因为需要开发方法，并且由于我建议生产的转录因子表达图将在选择全基因组分析的因素以及解释所得数据时提供一个关键的集成平台。与公共卫生的相关性：有关转录因子如何在发展中起作用的知识可能会提供有关相同转录因子在不当激活时如何在癌症中起作用的信息。此外，在这项研究中鉴定出的基因的人类直系同源物受到发育重要的转录因子的调节，可能在正常发育和疾病中起着作用。