Retroviral Transduction Core

逆转录病毒转导核心

• 批准号: 7313738
• 负责人: WARREN S PEAR
• 金额: $ 10.25万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7313738
• 关键词: Bone Marrow; Bone Marrow Transplantation; Cultured Cells; Ensure; Equipment; Fetal Liver; Fetal Thymic Organ Culture; Hematopoietic; Hematopoietic stem cells; Housing; Monitor; Mus; Mycoplasma; Nature; Pear; Preparation; Procedures; Production; Protocols documentation; Reagent; Research; Retroviridae; Standardization; Technical Expertise; Techniques; Training; discount; programs; retroviral transduction; small hairpin RNA; vector

Many of the experimental protocols for the projects in this program rely on retroviral transduction of primary or cultured cells. Because the techniques of retroviral preparation and transduction require a high degree of technical expertise, a central facility where these techniques are performed would significantly enhance the progress of all projects. A central facility for retroviral preparation and transduction would offer multiple advantages to the group. These include: 1) uniform procedures for preparing high titer retroviral supernatants, 2) regular availability of high titer retroviral supernatants, 3) periodic monitoring of retroviral supernatants to insure that they are mycoplasma-free and do not contain replication competent retrovirus, and 4) expertise in transduction of fetal liver and bone marrow hematopoietic stem cells, Because of the collaborative nature of the research program, availability of high titer retroviral supernatants and procedures for transduction, together with standardization of protocols and reagents would be of great benefit to everyone in the program. The retroviral core will be housed in Dr. Pear's research space. The Core will purchase and maintain equipment necessary for these procedures. Additionally, consumables will also be purchased in bulk by the Core to ensure standardization of materials and bulk purchase discounts.

该计划中项目的许多实验方案都依赖于主要的逆转录病毒转导 或培养的细胞。因为逆转录病毒制剂的技术需要高度的 技术专长，一种执行这些技术的中央设施将显着增强 所有项目的进展。逆转录病毒准备和转移的中央设施将提供多个 小组的优势。其中包括：1）准备高滴度逆转录病毒的均匀程序 上清液，2）定期可用逆转录病毒上清液，3）逆转录病毒的定期监测 上清液确保它们不含支原体，并且不包含复制能力逆转录病毒， 4）由于胎儿肝脏和骨髓造血干细胞的转导专业知识，因为 研究计划的协作性质，高滴水逆转录病毒上清液和程序的可用性 对于转导，以及协议和试剂的标准化，将有很大的好处 计划中的每个人。逆转录病毒核心将安置在Pear博士的研究空间中。核心意愿 购买并维护这些程序所需的设备。此外，消耗品也将是 由核心批量购买，以确保材料和大量购买折扣的标准化。