Rod-Tether Nanoprobe

杆系纳米探针

• 批准号: 7592921
• 负责人: Thomas Schneider
• 金额: $ 25.49万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7592921
• 关键词: Antibodies; Binding; Biological Assay; Biological Warfare; Blood; Class; Clinic; Clinical; Complex; Detection; Devices; Disease; Energy Transfer; Enzyme-Linked Immunosorbent Assay; Goals; Hereditary Disease; Legal patent; Light; Lighting; Messenger RNA; Microfluidics; Modification; Molecular; Oligonucleotides; Patients; Proteins; Pump; Range; Reaction; Reagent; Reporter; Resources; Signal Transduction; System; Time; Upper arm; cost; design; fluorophore; functional group; nanoparticle; nanoprobe; new technology; prototype; retinal rods; single molecule

The project is described at http://www.ccrnp.ncifcrf.gov/toms/patent/nanoprobe/ Rod-tether Nanoprobes are devices consisting of a rigid molecular rod with a flexible molecular tether attached at each end that can be used to detect and/or modify molecules. Each tether tip has a functional group, such as an antibody or oligonucleotide, that recognizes a target molecule. In addition, one tip carries a donor fluorophore and the other carries an acceptor fluorophore. The fluorophores form a pair for Forster Resonance Energy Transfer (FRET). In the absence of the target molecule, the rod keeps the tether arms apart most of the time, while in the presence of the target molecule, both recognizers bind to the target. This holds the donor and acceptor fluorophores close together. Illumination with light excites the donor and the energy is transferred by FRET to the nearby acceptor, which emits a detectable signal. By reducing an ELISA-like assay entirely to the molecular level, complex macroscopic or microfluidic washing and pumping systems can be eliminated. Rod-tether Nanoprobes can detect a wide variety of clinical and biowarfare reagents. The nanoprobes can also be used to rapidly and simply detect, modify and/or destroy endogenous molecules such as proteins and mRNA involved in a broad range of diseases. The simplest ssDNA-detecting nanoprobe has been created. Resources are sought to develop a variety of prototypes. In this past year, variety of new nanoprobe designs were developed.

该项目的描述见 http://www.ccrnp.ncifcrf.gov/toms/patent/nanoprobe/ Rod-tether 纳米探针是由刚性分子棒组成的装置，两端附有柔性分子系绳，可用于检测和/或修饰分子。每个系绳尖端都有一个功能组，例如识别目标分子的抗体或寡核苷酸。此外，一个尖端携带供体荧光团，另一个尖端携带受体荧光团。荧光团形成一对用于福斯特共振能量转移 (FRET)。在不存在目标分子的情况下，杆在大多数情况下使系绳臂保持分开，而在存在目标分子的情况下，两个识别器都与目标结合。这使得供体和受体荧光团紧密结合在一起。光照射激发供体，能量通过 FRET 转移到附近的受体，受体发出可检测的信号。通过将类似 ELISA 的测定完全降低到分子水平，可以消除复杂的宏观或微流体清洗和泵送系统。杆系纳米探针可以检测多种临床和生物战试剂。纳米探针还可用于快速、简单地检测、修饰和/或破坏内源性分子，例如与多种疾病有关的蛋白质和mRNA。最简单的单链 DNA 检测纳米探针已经诞生。寻求资源来开发各种原型。在过去的一年里，开发了多种新的纳米探针设计。