HIV-envelope-specific CD4+ T-cell activation and functional potentials

HIV 包膜特异性 CD4 T 细胞激活和功能潜力

• 批准号: 7495294
• 负责人: JULIA L HURWITZ
• 金额: $ 42万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-26 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7495294
• 关键词: Activated Lymphocyte; Adoptive Transfer; Animals; Antibody Formation; B-Lymphocytes; CD4 Positive T Lymphocytes; CD8B1 gene; Cell physiology; Cells; Clinical Trials; Data; Development; HIV-1; HIV-1 vaccine; Homing; Human; Immune; Immune response; Immune system; Infection; Killer Cells; Lymphocyte Function; Maintenance; Mediating; Mediator of activation protein; Monoclonal Antibodies; Mus; Phenotype; Population; Receptor Activation; Research; T-Cell Activation; T-Lymphocyte; Vaccine Design; Vaccines; Virus; Work; base; cytokine; design; improved; in vivo; inhibitor/antagonist; killings; mouse model; perforin; prevent; recombinant virus; research and development; research study

Despite decades of research, the development of a successful HIV-1 vaccine has not yet been achieved. A better understanding of the functions of activated lymphocytes is therefore desired. The long-term objective of our research is to comprehend the full potentials of HIV-1-envelope-specific immune cells. CD4+ T-cells contribute to HIV-1 control by supporting antibody production by 8-cells and the activation/maintenance of CD8+ T-cells. However, based on our recent data, it appears that envelope-specific CD4+ T-cells may additionally contribute directly to the control of virus-infected cells, independent of 8-cell or CD8+ T-cell activity. The studies proposed here will determine how these CD4+ T-cells confer their 'protector' effect. Specific Aim: To determine the phenotype, cytokine secretion capacities, and killer potentials of the HIV-1 envelope-specific CD4+ T-cells that protect against envelope-recombinant virus in the absence of 8-cell or CD8+ T-cell functions. Experiments are designed to fill fundamental gaps in our understanding of how virus is controlled by the immune system. Results from these experiments may be invaluable to the construction of new, successful HIV-1 vaccines designed to capture the full potentials of the immune response.

尽管经过数十年的研究，HIV-1 疫苗的成功开发尚未成功 实现了。因此，需要更好地了解活化淋巴细胞的功能。长期来看 我们研究的目的是了解 HIV-1 包膜特异性免疫细胞的全部潜力。 CD4+ T 细胞通过支持 8 细胞和 CD8+ T 细胞的激活/维持。然而，根据我们最近的数据，似乎特定于信封的 CD4+ T 细胞还可能直接有助于控制病毒感染的细胞，独立于 8 细胞或 CD8+ T 细胞活性。这里提出的研究将确定这些 CD4+ T 细胞如何赋予它们的“保护者” 影响。 具体目标：确定 HIV-1 的表型、细胞因子分泌能力和杀伤潜力 包膜特异性 CD4+ T 细胞，在缺乏 8 细胞或 CD8+ T 细胞功能。 实验旨在填补我们对病毒如何控制的理解的根本空白。 免疫系统。这些实验的结果对于构建新的、成功的 HIV-1 疫苗旨在充分发挥免疫反应的潜力。