Mechanisms Controlling BubR1 Regulation of Cancer and Aging

BubR1 调节癌症和衰老的控制机制

• 批准号: 9897456
• 负责人: Brian J. North
• 金额: $ 12.39万
• 依托单位: CREIGHTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-30 至 2021-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9897456
• 关键词: Acetylation; Address; Age; Aging; Anaphase; Aneuploidy; Animals; Automobile Driving; Birth; Caloric Restriction; Cell Aging; Cells; Chromosome Segregation; Chromosomes; Deacetylase; Deacetylation; Development; Disease; Engineering; Ensure; Exhibits; Genetic Materials; Goals; Human; In Vitro; Incidence; Individual; Light; Link; Longevity; Malignant Neoplasms; Mammals; Mediating; Metaphase Plate; Mitosis; Mitotic; Modification; Molecular; Mus; Mutation; Pathway interactions; Pharmacology; Phenotype; Phosphorylation; Physiological; Play; Post-Translational Protein Processing; Predisposition; Premature aging syndrome; Process; Protein-Serine-Threonine Kinases; Proteins; Regulation; Risk Factors; Rodent Model; Role; Signal Pathway; Therapeutic; Tissues; Tumor Suppressor Proteins; Ubiquitination; age related; aged; cancer therapy; genome integrity; in vivo; mouse model; overexpression; pleiotropism; prevent; segregation; senescence; therapeutic target; tumor; tumorigenesis

Abstract: The goal of this proposal is to elucidate the molecular mechanisms governing BubR1 protein abundance and function, and its role in the regulation of tumorigenesis and aging. Aging is the single greatest risk factor for cancer development, yet the mechanistic basis driving this interrelationship remains largely undefined. BubR1, a serine/threonine protein kinase, is involved in the spindle assembly checkpoint (SAC) to ensure faithful chromosome segregation during mitosis, and therefore is intimately linked to genomic integrity and cancer. Interestingly, recent studies have implicated BubR1 in the aging process where BubR1 abundance has been shown to decline in a variety of tissues as mammals age. Mice engineered to express low levels of BubR1 from birth die within a year, exhibiting increased senescence, premature aging phenotypes and an increased susceptibility to cancer, whereas mice overexpressing BubR1 have an extended lifespan with reduction in age- related diseases and cancer development. Senescence is believed to be largely tumor-suppressive and prevent cancer in young individuals. However, in aged individuals senescent cells can contribute to age-related cancer development. Therefore, BubR1 may play a pivotal role in the interrelationship between aging and cancer given that BubR1 suppresses both senescence and tumorigenesis. Previously, we identified an acetylation-dependent mechanism regulating BubR1 protein stability, where SIRT2 prevents degradation of BubR1 through deacetylation, leading to lifespan extension of a BubR1 premature aging mouse model. Furthermore, BubR1 protein levels in aged animals can be restored to youthful levels by stimulating SIRT2 activity through induction of NAD+ levels. These results suggest that the age-related decline in BubR1 levels can be reversed, potentially alleviating age-related diseases including cancer. Therefore, we hypothesize that BubR1 is a key tumor suppressor, and its loss with age increases cancer susceptibility. In this proposal, we plan to: 1) elucidate the physiological role of BubR1 post translational modifications during aging and calorie restriction and their impact on mitotic progression and tumorigenesis; and 2) determine the mechanisms regulating BubR1 protein abundance and function during aging. These studies will elucidate how regulation of BubR1 by post-translational modifications controls mitotic progression and tumorigenesis during aging as well as to identify mechanisms through which BubR1 declines with age and controls the aging processes. Given that aging poses the largest single risk factor for developing cancer, elucidating the molecular details governing the physiological role of BubR1 in cancer and aging will provide mechanistic understanding of the interrelationship between aging and cancer development, as well as identify possible therapeutic strategies to treat age-related diseases.

抽象的： 该提案的目标是阐明控制 BubR1 蛋白丰度和 功能及其在肿瘤发生和衰老调节中的作用。衰老是最大的单一风险因素 癌症的发展，但驱动这种相互关系的机制基础在很大程度上仍不清楚。布布R1, 丝氨酸/苏氨酸蛋白激酶，参与纺锤体组装检查点 (SAC)，以确保忠实 有丝分裂期间染色体分离，因此与基因组完整性和癌症密切相关。 有趣的是，最近的研究表明 BubR1 与衰老过程有关，其中 BubR1 丰度一直在 随着哺乳动物年龄的增长，多种组织的功能都会下降。小鼠经过基因改造以表达低水平的 BubR1 从出生起一年内就会死亡，表现出衰老增加、早衰表型和衰老增加 癌症易感性，而过度表达 BubR1 的小鼠寿命延长，年龄降低 相关疾病和癌症的发展。衰老被认为在很大程度上具有肿瘤抑制作用 预防年轻人的癌症。然而，在老年人中，衰老细胞可能会导致与年龄相关的疾病 癌症的发展。因此，BubR1可能在衰老与衰老之间的相互关系中发挥着关键作用。 鉴于 BubR1 可以抑制衰老和肿瘤发生。之前，我们确定了一个 乙酰化依赖性机制调节 BubR1 蛋白稳定性，其中 SIRT2 防止 BubR1 蛋白降解 BubR1 通过脱乙酰化，导致 BubR1 早衰小鼠模型的寿命延长。 此外，通过刺激 SIRT2，老年动物的 BubR1 蛋白水平可以恢复到年轻水平 通过诱导 NAD+ 水平来发挥活性。这些结果表明 BubR1 水平与年龄相关的下降 可以逆转，有可能减轻包括癌症在内的与年龄相关的疾病。因此，我们假设 BubR1 是一种关键的肿瘤抑制因子，随着年龄的增长，它的丧失会增加癌症的易感性。在这个提案中，我们 计划：1) 阐明 BubR1 翻译后修饰在衰老和卡路里摄入过程中的生理作用 限制及其对有丝分裂进展和肿瘤发生的影响； 2）确定机制 在衰老过程中调节 BubR1 蛋白丰度和功能。这些研究将阐明如何监管 BubR1 通过翻译后修饰控制衰老过程中的有丝分裂进展和肿瘤发生 以确定 BubR1 随着年龄增长而下降并控制衰老过程的机制。给定 衰老是罹患癌症的最大单一风险因素，阐明了控制癌症的分子细节 BubR1 在癌症和衰老中的生理作用将为我们提供机制理解 衰老与癌症发展之间的相互关系，以及确定可能的治疗策略 治疗与年龄有关的疾病。

项目成果

Cullin 3SPOP ubiquitin E3 ligase promotes the poly-ubiquitination and degradation of HDAC6.

Cullin 3SPOP 泛素 E3 连接酶促进 HDAC6 的多泛素化和降解。

• 发表时间: 2017-07-18
• 作者: Tan, Yuyong;Ci, Yanpeng;Dai, Xiangpeng;Wu, Fei;Guo, Jianping;Liu, Deliang;North, Brian J;Huo, Jirong;Zhang, Jinfang
• 通讯作者: Zhang, Jinfang

Unraveling the Molecular Nexus between GPCRs, ERS, and EMT.

揭示 GPCR、ERS 和 EMT 之间的分子关系。

• 发表时间: 2021
• 影响因子: 4.6
• 作者: Kumari, Niti;Reabroi, Somrudee;North, Brian J
• 通讯作者: North, Brian J

Physiological relevance of post-translational regulation of the spindle assembly checkpoint protein BubR1.

纺锤体组装检查点蛋白 BubR1 翻译后调节的生理相关性。

• 发表时间: 2021-04-23
• 作者: Bloom, Celia R;North, Brian J
• 通讯作者: North, Brian J

Regulation of topoisomerase II stability and activity by ubiquitination and SUMOylation: clinical implications for cancer chemotherapy.

通过泛素化和 SUMO 化调节拓扑异构酶 II 稳定性和活性：癌症化疗的临床意义。

• 发表时间: 2021-09
• 影响因子: 2.8
• 作者: Ma, Ying;North, Brian J;Shu, Jianfeng
• 通讯作者: Shu, Jianfeng