Histology and Imaging Core

组织学和成像核心

• 批准号: 7746214
• 负责人: Christopher C Norbury
• 金额: $ 22.47万
• 依托单位: RESEARCH INST OF FOX CHASE CAN CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7746214
• 关键词: Animals; Area; Arts; Biohazardous Substance; Biological Response Modifiers; CD4 Positive T Lymphocytes; Cells; Chickenpox; Complex; Containment; Core Facility; Data; Dendritic Cells; Dependence; Disease; Enterovirus; Equipment; Event; Exposure to; Flavivirus; Fostering; Health; Histology; Housing; Human; Human Resources; Image; Image Analysis; Imagery; Immune; Immune system; Individual; Infection; Infectious Ectromelia; Infiltration; Institutes; Interferon Type I; Investigation; Laboratories; Life; Liver; MHC Class II Genes; Maintenance; Measles; Methods; Microscopic; Microscopy; Morbillivirus; Mouse Pox Virus; Mumps; Mus; Optics; Pathway interactions; Peripheral; Poliomyelitis; Poxviridae; Preparation; Process; Relative (related person); Research; Research Personnel; Research Project Grants; Role; Route; Rubella; Rubivirus; Rubulavirus; Sampling; Skin; Smallpox; Specimen; Spleen; Staining method; Stains; Techniques; Technology; Time; Tissue Stains; Tissues; Virion; Virus; Virus Diseases; Virus Replication; West Nile virus; Yellow Fever; cost; cryostat; design; digital imaging; experience; foot; image processing; insight; instrumentation; lymph nodes; meetings; optical imaging; pathogen; prevent; programs; research study; response; solute; trafficking

The Projects within this Center study the interplay between Ectromelia virus (ECTV), the causative agent of mousepox (the smallpox of the mouse), and its natural host, the mouse. The interplay between ECTV and the immune system of the host can take place at many different levels. It can occur at a tissue level, in which release of immune mediators such as type I interferons alter infection in the skin (Project 1) or lymph node, spleen and liver (Project 2) by ECTV., It can also occur at the cellular level, in which immune cell infiltration and interaction with ECTV infected cells will alter virus replication and spread (Project 1, 3). Finally, it can occur at an intracellular level, in which the trafficking of internalized ECTV, and the endocytic routes used during ECTV infection, can alter the mechanisms by which the immune system senses infection (Project 3 + Aim 2 of this Core). This Core (C) will allow the visualization of all of these events, by providing timely, cost-effective and expert histology and microscopy support for all three Projects within the Center. The equipment and expertise that will be provided by this Core are aimed at reducing the startup time and degree of expertise that would be necessary for individual users if they were required to design and execute experiments and image processing. In addition, the Core will allow imaging of live virus in Biosafety Level 2 containment, which is not possible in shared facilities. In addition to providing support for all three Projects, Aim 2 of this Core will investigate an area of research that bridges areas of investigation in Projects 1 and 3, namely the endocytic processes involved in ECTV internalization and infection, and the presentation of virus-derived components in complex with MHC Class II. Thus, the Core will enhance both the scientific capabilities of the Center and will foster interactions between the individual Projects.

该中心内的项目研究了Ectromelia病毒（ECTV）（病因剂）之间的相互作用 Mousepox（小鼠的天花）及其天然宿主小鼠。 ECTV之间的相互作用 宿主的免疫系统可以在许多不同的水平上进行。它可以在组织水平上发生， 在其中释放免疫介质（I型干扰素）等免疫介质会改变皮肤中的感染（项目1）或 淋巴结，脾脏和肝（项目2）通过ECTV。，它也可以在细胞水平上发生，其中免疫。 细胞浸润和与ECTV感染细胞的相互作用将改变病毒复制和扩散（项目1，3）。 最后，它可以发生在细胞内，其中内部ECTV的贩运和内吞细胞的运输 ECTV感染期间使用的路线可以改变免疫系统感官的机制 感染（该核心的项目3 +目标2）。该核心（c）将允许所有这些事件可视化， 为及时，具有成本效益和专家的组织学和显微镜支持，为所有三个项目提供 中心。该核心将提供的设备和专业知识旨在减少初创企业 如果需要设计的时间和专业知识，则需要设计的时间和程度 并执行实验和图像处理。此外，核心将允许在 生物安全2级遏制，在共享设施中不可能。除了提供支持 这三个项目，该核心的目标2将调查一个研究领域，该领域桥接了调查领域 在项目1和3中，即ECTV内部化和感染涉及的内吞过程，以及 与MHC II类复杂的病毒衍生成分的呈现。因此，核心将增强 该中心的科学能力都将促进各个项目之间的互动。