ELECTRON MICROSCOPY CORE

电子显微镜核心

• 批准号: 7318795
• 负责人: VIRGINIA M PICKEL
• 金额: $ 10.22万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7318795
• 关键词: Anatomy; Antigens; Arts; Basic Science; Brain; Brain region; Detection; Electron Microscope; Electron Microscopy; Electrons; Equipment; Faculty; Floor; Freezing; Glass; Goals; Human Resources; Image; Imaging Techniques; Immune Sera; In Situ Hybridization; Investigation; Medical; Messenger RNA; Microscopic; Microscopy, Electron, Other; National Institute of Drug Abuse; Neuraxis; Neurology; Neurosciences; Pilot Projects; Plant Resins; Proteins; Research; Resources; Scientist; Services; Slide; Standards of Weights and Measures; Techniques; Technology; Tissues; Training; Universities; addiction; college; member; new technology; novel; receptor; tissue processing; trafficking

The Electron Microscopy (EM) Core is a critical component of the NIDA sponsored addiction center, which provides resources and services contributing to the cellular and subcellular localization of transmitters, receptors and transporters under investigation. These are important for all the basic science research in Projects 1-3, and crucial for the quantitative electron microscopic immunolabeling in Project 3. The core personnel include two faculty members of the Department of Neurology and Neuroscience, Weill Medical College of Cornell University. The Director, Dr. Pickel, has pioneered and established many facets of preembedding EM immunolabeling of a variety of antigens in the central nervous system, and also has substantial expertise in brain anatomy and LM detection of mRNA and proteins under investigation in Projects 1 and 2. The co-director, Dr. Glass has made significant contributions to the use of quantitative morphometric analysis of receptor trafficking under the influence of addictive substances. The resources available in the core include a CM-10 and Techni electron microscopes as well as all smaller equipment seeded for LM and EM structural analysis. The core will provide services and facilities related to (a) characterization of antisera and processing of the tissue for both LM and EM immunolabeling either before (pre-) or after (post-) embedding in resin to for microscopic analysis, (b) in situ hybridization of mRNA on frozen slide-mounted brain sections, (c) image acquisition and quantification of immunolabeling. The Core faculty and resources are located at Weill Medical College of Cornell University, which is across the street from Rockefeller University, and occupies one floor of the Kips Bay building. Access to this facility is easily gained by Rockefeller University associates with the presentation of proper identification. The Goals of the EM Resourse are: 1. To offer state of the art ultrastructural examination of tissues from specific brain region, utilizing standard and novel forms of electron microscopy and other standard imaging techniques, for the Center, including Project 3, possibly Projects 1 and 2, and any pilot projects which require this technology. 2. To develop new technologies for ultrastructural imaging as needed. 3. To train scientists and technical support staff in the various techniques of electron microscopy and related imaging techniques.

电子显微镜（EM）核心是NIDA赞助成瘾中心的关键组成部分，该中心是 提供有助于发射器的细胞和亚细胞定位的资源和服务， 受体和转运蛋白正在研究。这些对于所有基础科学研究都很重要 项目1-3，对于项目3中的定量电子显微镜免疫标记至关重要。核心 人员包括神经病学和神经科学系的两名教职员工，Weill Medical 康奈尔大学学院。董事Pickel博士开创并建立了许多曾经构成的方面 中枢神经系统中各种抗原的EM免疫标记，也具有 研究正在研究的mRNA和蛋白质的大脑解剖学和LM检测方面的大量专业知识 项目1和2。格拉斯博士联合导演为使用定量做出了重大贡献 在成瘾性物质的影响下对受体运输的形态分析。资源 核心中可用包括CM-10和技术电子显微镜以及所有较小的设备 播种进行LM和EM结构分析。核心将提供与（a）相关的服务和设施 在以前的LM和EM免疫标记的抗血清和组织的加工表征 （前）或（后）嵌入树脂中以进行微观分析，（b）原位mRNA的原位杂交 冷冻幻灯片安装的脑部，（c）免疫标记的图像采集和定量。核心 教职员工和资源位于康奈尔大学的威尔医学院，该学院在街对面 来自洛克菲勒大学，占据了基普斯湾大楼的一层。进入该设施很容易 洛克菲勒大学获得了适当的身份证明。 EM资源的目标是： 1。使用标准，提供特定大脑区域的组织的超微结构检查 以及新型电子显微镜和其他标准成像技术的新形式，包括 项目3，可能是项目1和2，以及任何需要此技术的试点项目。 2。根据需要开发用于超微结构成像的新技术。 3。培训科学家和技术支持人员的电子显微镜和相关技术的各种技术 成像技术。