Cell-specific and multifunctional drug formulations for in vivo delivery

用于体内递送的细胞特异性和多功能药物制剂

• 批准号: 9483331
• 负责人: Yizhou Dong
• 金额: $ 38.5万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9483331
• 关键词: Address; Animal Model; Animals; Antibodies; Antineoplastic Agents; Apoptosis; Binding; Biomedical Engineering; Blood - brain barrier anatomy; Brain; Brain Neoplasms; Breast Cancer Cell; Cells; Chlorotoxin; Communicable Diseases; Cytotoxic agent; Detection; Disease; Drug Delivery Systems; Drug Formulations; Engineered Gene; Epithelial Cells; Excision; Formulation; Gelatinase A; Gene Silencing; Genetic Diseases; Goals; Guide RNA; Hemophilia A; Hepatitis B; Human; Image; Knowledge; Ligands; Lung; Malignant Neoplasms; Mediating; Messenger RNA; Metastatic breast cancer; Molecular Probes; Peptides; Pharmaceutical Chemistry; Pharmaceutical Preparations; Pharmacy (field); Proteins; Research; Respiratory syncytial virus; Small Interfering RNA; Structure; System; Translating; Treatment Efficacy; Viral Genome; Virus; Virus Diseases; cancer therapy; cell type; cyanine; design; image guided; imaging probe; in vivo; innovation; invention; medical specialties; nanoparticle; neoplastic cell; novel therapeutics; nucleolin; overexpression; programs; receptor; small molecule; tool; tumor; tumor specificity; uptake

PROJECT SUMMARY The objectives of this project are 1) to develop cell-specific drug delivery systems; 2) to construct multifunctional drug delivery systems; and 3) to demonstrate therapeutic efficacy of these systems in animal models for treating genetic disorders, infectious diseases, as well as cancers. Cell-specific delivery in vivo is one of the most challenging issues in the field of drug delivery. To address this issue, we propose two strategies: (1) to mimic the receptor-mediated virus entry. The simple structure and composition of viruses make them capable of specifically infecting many cell types in humans. Our research program will apply knowledge of viral infections in order to guide the design of cell-specific delivery systems. For example, nucleolin has been identified as a cellular receptor on epithelial cells for human respiratory syncytial virus (RSV) in the lungs. We will utilize anti-nucleolin antibody or nucleolin ligands in order to target lung epithelial cells. (2) to target tumor over-expressed receptors. Previous studies, however, have identified a number of tumor over-expressed receptors, of which many are relatively unexplored and our understanding of them is very limited. For example, Chlorotoxin, a short peptide, is capable of crossing the blood brain barrier (BBB) and binding specifically to the matrix metalloproteinase-2 (MMP-2), which is over-expressed in brain tumor cells. We propose to utilize these ligands in order to specifically target tumor cells. We will evaluate their tumor specificity with imaging probes. In order to develop multifunctional drug delivery systems, we will integrate the ligands identified from the above studies with different delivery systems such as nanoparticles and conjugates. The payloads can be mRNA, siRNA, and small-molecule drugs to yield multifunctional delivery systems. The delivery systems are capable of utilizing mRNA for expressing functional proteins, siRNA for gene silencing, and small-molecule drugs for cancer therapy. By delivering mRNA encoding Cas9 and specific guide RNA (sgRNA), we anticipate achieving gene engineering in vivo in order to find a cure for genetic disorders such as hemophilia and to deplete viral genome for treating viral infections such as hepatitis B. In addition, we have designed Chlorotoxin-Cyanine-Drug (CCD) as a multifunctional conjugate in order to specifically deliver near- infrared molecular probes and cytotoxic agents to brain metastatic breast cancer (BMBC) tumor cells. After administration, CCD conjugates will cross the BBB and bind with MMP-2 on BMBC cells, triggering the cellular uptake of the CCD. Cyanine probes will facilitate imaging-guided tumor resection. Meanwhile, cytotoxic agents will induce the apoptosis of tumor cells, which are below detection limits of imaging and for which surgical removal is not a possibility. Our research goal is to translate the innovation of this research strategy in order to develop better drug delivery tools to treat diverse diseases.

项目概要 该项目的目标是1）开发细胞特异性药物输送系统； 2）构建 多功能药物输送系统； 3) 证明这些系统在动物身上的治疗效果 治疗遗传性疾病、传染病和癌症的模型。体内细胞特异性递送是 药物输送领域最具挑战性的问题之一。为了解决这个问题，我们提出两个建议 策略：（1）模仿受体介导的病毒进入。病毒的简单结构和组成 使它们能够特异性感染人类的​​多种细胞类型。我们的研究计划将适用 病毒感染的知识，以指导细胞特异性递送系统的设计。例如， 核仁素已被鉴定为人呼吸道合胞病毒上皮细胞上的细胞受体 (RSV) 在肺部。我们将利用抗核仁素抗体或核仁素配体来靶向肺上皮细胞 细胞。 (2)针对肿瘤过度表达的受体。然而，之前的研究已经确定了一些 肿瘤过度表达的受体，其中许多受体相对尚未被探索，我们对它们的理解是 非常有限。例如，氯毒素（Chlorotoxin）是一种短肽，能够穿过血脑屏障（BBB）并 特异性结合基质金属蛋白酶-2 (MMP-2)，该酶在脑肿瘤细胞中过度表达。 我们建议利用这些配体来特异性靶向肿瘤细胞。我们将评估他们的肿瘤 成像探针的特异性。为了开发多功能药物输送系统，我们将整合 从上述研究中鉴定出的配体具有不同的递送系统，例如纳米颗粒和缀合物。 有效负载可以是 mRNA、siRNA 和小分子药物，以产生多功能递送系统。这 递送系统能够利用 mRNA 表达功能性蛋白质，利用 siRNA 进行基因沉默， 以及用于癌症治疗的小分子药物。通过传递编码Cas9的mRNA和特定的向导RNA （sgRNA），我们期望在体内实现基因工程，以便找到治疗遗传性疾病的方法，例如 血友病并消除病毒基因组以治疗乙型肝炎等病毒感染。此外，我们还有 设计了氯毒素-花青-药物（CCD）作为多功能缀合物，以特异性地传递近- 针对脑转移性乳腺癌 (BMBC) 肿瘤细胞的红外分子探针和细胞毒剂。后 给药后，CCD缀合物将穿过BBB并与BMBC细胞上的MMP-2结合，触发细胞 CCD 的吸收。花青探针将有助于成像引导的肿瘤切除。同时，细胞毒剂 会诱导肿瘤细胞凋亡，这些细胞凋亡低于成像检测限，需要手术治疗 移除是不可能的。我们的研究目标是将这一研究策略的创新转化为 开发更好的药物输送工具来治疗多种疾病。