SUGARS AS NOVEL CRYOPROTECTANTS FOR PRIMATE OOCYTES

糖作为灵长类卵母细胞的新型冷冻保护剂

• 批准号: 7562669
• 负责人: KENNETH G GOULD
• 金额: $ 3.16万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562669
• 关键词: Biological Preservation; Computer Retrieval of Information on Scientific Projects Database; Cryoprotective Agents; Development; Embryo; Female; Fertilization; Fertilization in Vitro; Freezing; Funding; Future; Genetic; Germ Cells; Grant; Hamsters; Hormonal; Infertility; Institution; Legal; Medical; Methods; Modeling; Oocytes; Operative Surgical Procedures; Primates; Procedures; Recovery; Reproduction; Research; Research Personnel; Resources; Source; Stress; Testing; Time; United States National Institutes of Health; Woman; Work; chemotherapy; novel; programs; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Assisted reproduction, including in vitro fertilization (IVF) is an important part of medical treatment for women who have difficulty conceiving naturally. IVF involves hormonal stimulation of the woman to induce multiple follicle development with surgical recovery of oocytes for fertilization. This procedure may have to be repeated on several occasions. Freeze preservation of female gametes would reduce the stress associated with repeated IVF. While it is practical to freeze preserve embryos, freeze preservation of primate oocytes has not been predictable successful. Embryo storage is not always the best option, for example when oocyte recovery prior to chemotherapy etc. would allow future use of IVF for a woman not associated with a partner at the time of treatment. In addition, certain legal problems associated with embryo freezing could be avoided. Oocyte preservation would also be of value in conservation programs for endangered species. This work tests the use of sugars as cryoprotective agents for primate oocytes in the primate model as there are genetic differences in oocytes which have precluded application of methods developed for storage of (for example) hamster oocytes.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 辅助繁殖，包括体外受精（IVF）是对自然构想的妇女的医疗治疗的重要组成部分。 IVF涉及对女性的激素刺激，以诱导多种卵泡发育，并通过手术恢复卵母细胞进行受精。 可能需要多次重复此过程。 冻结女配子的保存将减少与重复IVF相关的应力。 虽然冻结保存胚胎是实际的，但冻结灵长类动物卵母细胞的保存尚不可预测。 胚胎储存并不总是最佳选择，例如，当化学疗法之前的卵母细胞恢复会允许在治疗时与与伴侣无关的女性使用IVF时。 此外，可以避免与胚胎冷冻相关的某些法律问题。 在濒危物种的保护计划中，卵母细胞保存也将具有价值。 这项工作测试了在灵长类动物模型中使用糖作为灵长类动物卵母细胞的冷冻保护剂，因为卵母细胞存在遗传差异，这些卵母细胞排除了用于存储（例如）仓鼠卵母细胞的方法的应用。