Role of physical training and intraspinal hemodynamic changes in the recovery of forelimb function after cervical spinal cord injury in rats

体能训练及椎管内血流动力学变化对大鼠颈髓损伤后前肢功能恢复的作用

• 批准号: 10783208
• 负责人: ZIN Z KHAING
• 金额: $ 5.91万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-15 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10783208
• 关键词: Acute; Award; Blood flow; Cervical spinal cord injury; Chronic; Control Groups; Data Analyses; Doctor of Philosophy; Educational Intervention; Experimental Designs; Forelimb; Future; Glyburide; Goals; Grant; Histological Techniques; Image; Imaging Techniques; International; Learning; Longitudinal Studies; Manuscripts; Mediating; Mentors; Mentorship; Modeling; Neuroprotective Agents; Operative Surgical Procedures; Parents; Physical therapy; Rattus; Recovery; Recovery of Function; Research; Research Project Grants; Rodent; Role; Scientist; Spinal Cord; Spinal cord injury; Sterility; Techniques; Testing; Training; Ultrasonography; Work; Writing; career; combinatorial; contrast enhanced; design; hemodynamics; improved; meetings; neurological rehabilitation; neuroprotection; novel; programs; skills; treatment group; ultrasound

Project Summary This request for an administrative diversity supplement award will allow Mr. Hernandez to develop as a scientist and gain additional training and skills needed to be competitive candidate into a PhD program in Physical Therapy. Mr. Hernandez will work with Dr. Khaing (mentor), Dr. Bruce (co-mentor), and Dr. Chet Moritz (co-mentor) to learn and develop skills to complete a successful research project. Mr. Hernandez proposed to develop a project examining 1) the combined effects of physical therapy and a neuroprotective agent (Glibenclamide), and 2) altered blood flow within the chronic spinal cord after cervical spinal cord injury. This project is within the scope of the parent R01 grant because it aims to explore how to obtain optimal functional recovery by combining neuroprotection (proposed in the parent R01) with physical therapy training. Additionally, this project will also utilize the novel ultrasound imaging technique proposed in the parent R01 to explore the alterations in intraspinal blood flow changes that may mediate or correlate with improved functional recovery in a rat model of spinal cord injury. We hypothesize that rats that receive physical training and Glibenclamide will be able to perform forelimb reaching tasks (FRT) more effectively when compared to other groups treated with physical training or Glibenclamide alone. Specifically, we will test whether functional recovery of forelimbs can be enhanced with a physical therapy/training intervention. Recently our lab has successfully developed contrast-enhanced ultrasound (CEUS) for use with rodent traumatic SCI. Here will utilize acute and transcutaneous B-mode and contrast enhanced ultrasound (CEUS) imaging to perform a longitudinal study in control and treatment groups to determine hemodynamic changes associated with improved functional recovery after cSCI. Through this proposed study, Mr. Hernandez will receive training on designing and executing a research project, sterile surgical techniques, ultrasound imaging techniques as well as histological techniques to accomplish the proposed work. Additionally. Mr. Hernandez will receive training in MATLAB for image acquisition, processing and data analysis using this program. Mr. Hernandez will receive mentoring from Drs. Khaing, Bruce, and Moritz on experimental design, execution as well as data analysis, interpretation, and manuscript writing skills. Mr. Hernandez will also be expected to present the findings from this project at a national or international meeting to gain additional exposure and networking opportunities. Finally, Mr. Hernandez will be encouraged to develop his own research ideas to be developed as F31 in the future.

项目概要 行政多元化补充奖的请求将使埃尔南德斯先生能够发展 作为一名科学家，并获得成为有竞争力的候选人所需的额外培训和技能 物理治疗博士课程。 Hernandez 先生将与 Khaing 博士（导师）、Bruce 博士一起工作 （共同导师）和 Chet Moritz 博士（共同导师）学习和发展技能以成功完成 研究项目。 Hernandez 先生提议开发一个项目来检查 1) 综合效应 物理治疗和神经保护剂（格列本脲），以及 2）改变体内的血流 颈脊髓损伤后的慢性脊髓。本项目属于本项目范围 母公司 R01 拨款，因为它旨在探索如何通过以下方式获得最佳功能恢复： 将神经保护（在母版 R01 中提出）与物理治疗训练相结合。 此外，该项目还将利用在 父母 R01 探索可能介导或的椎管内血流变化的改变 与脊髓损伤大鼠模型的功能恢复改善相关。我们假设 接受体能训练和格列本脲的老鼠将能够进行前肢伸展 与接受体育训练或治疗的其他组相比，任务（FRT）更有效 单独使用格列本脲。具体来说，我们会测试前肢的功能能否恢复。 通过物理治疗/训练干预得到增强。最近我们实验室成功 开发了用于啮齿类动物创伤性 SCI 的对比增强超声 (CEUS)。这里将 利用急性经皮 B 型和对比增强超声 (CEUS) 成像 在对照组和治疗组中进行纵向研究以确定血流动力学 与 cSCI 后功能恢复改善相关的变化。通过这个提议 研究中，埃尔南德斯先生将接受设计和执行研究项目的培训，无菌 手术技术、超声成像技术以及组织学技术 完成拟议的工作。此外。 Hernandez 先生将接受 MATLAB 培训 使用该程序进行图像采集、处理和数据分析。埃尔南德斯先生将收到 博士的指导。 Khaing、Bruce 和 Moritz 关于实验设计、执行和数据 分析、解释和手稿写作技巧。预计埃尔南德斯先生还将 在国家或国际会议上展示该项目的研究结果以获得更多信息 曝光和交流机会。最后，将鼓励埃尔南德斯先生发展他的 自己的研究思路未来将发展为F31。