Imaging spatial transcriptome at single cell resolution to study eye regeneration in old age

以单细胞分辨率对空间转录组进行成像以研究老年眼再生

• 批准号: 10785595
• 负责人: Longhua Guo
• 金额: $ 19.5万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-30 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10785595
• 关键词: Age; Age related macular degeneration; Aging; Animal Model; Animals; Architecture; Atlases; Body part; Cell Communication; Cells; Collaborations; Data; Data Set; Development; Disease; Elderly; Eye; Fresh Water; Gene Expression; Genes; Goals; Head; Image; Impairment; Knowledge; Laboratory culture; Learning; Methods; Molecular; Natural regeneration; Output; Pathway interactions; Patients; Pattern; Persons; Phenotype; Pigments; Planarians; Platyhelminths; Process; Production; Quality of life; Regenerative Medicine; Regenerative capacity; Regulation; Rejuvenation; Reporting; Research; Resolution; Signal Transduction; Slide; Technology; Therapeutic; Tissues; Work; adult stem cell; age related; aged; cell type; differential expression; empowerment; experimental study; eye regeneration; fighting; human old age (65+); improved; molecular dynamics; next generation; older patient; progenitor; regenerative; repaired; single cell mRNA sequencing; stem cell biology; stem cell function; stem cell technology; stem cells; theories; tissue regeneration; tool; transcriptome; transcriptomics

ABSTRACT To cure age-related diseases is of critical importance for the life quality of elderly people. One promising strategy is to slow down aging or reverse aging with regenerative medicine. However, the regenerative capacity of stem cells and their supportive microenvironments are drastically impaired in old age. Hence, to rejuvenate functions of aged adult stem cells and their microenvironments or to maintain the youthful functions of adult stem cells in old age are critical research directions to empower regenerative medicine to fight age-associated diseases. Here, we propose to use a cutting-edge spatial transcriptomics method, Seq-Scope, to comprehensively examine the aging, regeneration and rejuvenation of eye progenitor cells and their niche cells in the sexual planarians, a champion of tissue regeneration. We aim to determine the molecular pathways that facilitate regeneration and rejuvenation in old age. The proposed experiments will define datasets of fundamental importance in the identification of niche cells and changes in aging and regeneration that will fuel long-term mechanistic studies.

抽象的 治愈与年龄有关的疾病对于老年人的生活质量至关重要。一个有希望的策略 是通过再生医学降低衰老或反向衰老。但是，茎的再生能力 细胞及其支撑性微环境在老年时严重受损。因此，恢复功能 成年干细胞及其微环境的老化或维持成年干细胞的年轻功能 老年是赋予再生医学能力与年龄相关疾病的关键研究方向。这里， 我们建议使用尖端的空间转录组学方法Seq-Scope，以全面检查 眼祖细胞及其小众细胞的老化，再生和恢复活力 组织再生的拥护者。我们旨在确定促进再生和 恢复活力。拟议的实验将定义具有基本重要性的数据集 鉴定利基细胞以及衰老和再生的变化，这将促进长期机理研究。