TAKASHIMA DIVERSITY SUPPLEMENT TO: REGULATION OF GAMETE USE AND NEURAL PATHWAYS IN REPRODUCTION

高岛多样性补充：配子使用和生殖神经通路的调节

• 批准号: 10764479
• 负责人: ANDREW G CLARK
• 金额: $ 8.97万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-24 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10764479
• 关键词: Academic Training; Affect; Area; Biological Assay; Biology; Data; Development; Doctor of Philosophy; Drosophila genus; Educational process of instructing; Educational workshop; Ejaculation; Encapsulated; Ethics; Female; Fertilization; Germ Cells; Goals; Immunofluorescence Microscopy; Latina; Learning; Lipid Binding; Mammals; Mentors; Modeling; Neural Pathways; Partner in relationship; Phenotype; Process; Production; Proteins; RNA; Regulation; Reproduction; Research; Research Training; Resources; Science; Seminal; Statistical Data Interpretation; Statistical Models; System; Training; Variant; Vesicle; Work; career; career development; doctoral student; exosome; experience; father role; genetic analysis; genetic variant; genome wide association study; graduate student; interest; male; meetings; reproductive; reproductive success; response; skills; sperm cell; student mentoring; symposium

Project Summary This is a Diversity Supplement to support the development of the career of Yoko Takashima, a second year PhD student who is engaged in work related to R01 HD059060, “Regulation of Gamete Use and Neural Pathways in Reproduction”. Her work focuses on three primary aims related to the determinants of reproductive success. The first aim is to elucidate the functional consequences of the transfer of exosomes (vesicles) from males to females during mating, a phenomenon seen in mammals and in Drosophila. The function of these transferred exosomes, minute lipid-bound vesicles that encapsulate and transfer proteins and RNAs, is not well- understood in any system. In Drosophila they have been suggested to affect the process of sperm competition, in which ejaculates from successive males compete for fertilization opportunities. Ms. Takashima will assess the variation in exosome production, transfer, and its effects on sperm competition, allowing her to apply genome-wide association (GWAS) approaches to identify previously unknown genetic variants that impact exosome biology. In her second aim, Ms. Takashima will dissect the mechanism(s) employed by exosome contents to impact female post-mating responses, including a specific assay of sperm competitive ability. Finally, in Aim 3, these data will be incorporated into a promising statistical modeling approach, the Bradley-Terry model, to quantify the effects of perturbed seminal exosomes on male competitive-reproductive success. We have devised a detailed mentoring plan that will provide Ms. Takashima tailored support for both her scientific development and to guide her in achieving her career goals. She will receive academic and research training, training in presentational skills and responsible conduct in research, teaching/mentoring experience, and guidance to complete an impactful PhD. She will also receive additional guidance to help her choose among career options that she is considering; this will involve one-on-one guidance from her mentors, networking that we will set up for her with colleagues and alumni in careers she is exploring, as well as workshops and resources at Cornell and at conferences she will attend.

项目摘要 这是一种多样性补充，旨在支持Yoko Takashima职业的发展 从事与R01 HD059060相关的工作的第二年博士生 配子使用和繁殖的神经途径”。她的工作重点介绍了三个主要目标 与生殖成功的决定者有关。第一个目的是阐明功能 在交配过程中，外泌体（囊泡）从男性转移到女性的后果 在哺乳动物和果蝇中看到的现象。这些转移的外泌体的功能， 封装和转移蛋白质和RNA的微小脂质结合的蔬菜不是很好 在任何系统中都了解。在果蝇中，他们被建议影响 精子竞赛，其中成功的男性射精竞争施肥 机会。 Takashima女士将评估外部生产，转移及其的变化 对精子竞争的影响，使她能够应用全基因组关联（GWAS） 识别影响外泌体生物学的以前未知的遗传变异的方法。在她里面 第二个目的，高岛女士将剖析外泌体内容所带来的机制 影响女性后的比赛反应，包括对精子竞争能力的特定评估。 最后，在AIM 3中，这些数据将被纳入有希望的统计建模方法中， Bradley-terry模型，以量化被扰动的第二个外泌体对男性的影响 竞争性生产成功。我们制定了一个详细的心理计划，将提供 高岛女士对她的科学发展量身定制支持，并指导她实现 她的职业目标。她将接受学术和研究培训，介绍培训 技能和负责任的研究，教学/指导经验以及指导 完成有影响力的博士学位。她还将获得其他指导，以帮助她选择 她正在考虑的职业选择；这将涉及她的导师一对一的指导， 我们将在她正在探索的职业中与同事和校友建立网络，因为 以及康奈尔（Cornell）的研讨会和资源，她将参加会议。