Integration of visual information and behavioral modulation in the superior colliculus

上丘视觉信息和行为调节的整合

• 批准号: 10737733
• 负责人: Elise L Savier
• 金额: $ 24.88万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2026-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10737733
• 关键词: Anatomy; Animal Model; Animals; Area; Arousal; Attention; Behavior; Behavioral; Brain; Brain region; Calcium; Cells; Dorsal; Electrophysiology (science); Emotional; Evolution; Genetic; Goals; House mice; Image; Instruction; Label; Lateral Geniculate Body; Locomotion; Methods; Midbrain structure; Modality; Molecular; Morphology; Motion; Mus; Neurons; Outcome; Pathologic; Pathway interactions; Pattern; Perception; Phase; Population; Positioning Attribute; Primates; Process; Property; Pulvinar structure; Reporting; Research; Research Personnel; Retina; Sampling; Schizophrenia; Sensory; Shapes; Source; Stream; Structure; System; Testing; Thalamic structure; Tupaia; Tupaiidae; Viral; Vision; Visual; Visual Cortex; Visual Fields; Visual Pathways; Visual System; Work; area striata; autism spectrum disorder; behavioral study; cell motility; cell type; comparative; experience; experimental study; novel strategies; optogenetics; preference; response; sensory input; superior colliculus Corpora quadrigemina; tool; visual information

PROJECT SUMMARY (See instructions): Distinct visual features are extracted in parallel to be later combined and modulated along the visual pathways. Two parallel pathways can be found early on, with projections from the retina targeting the dorsal lateral geniculate nucleus, which targets cortical areas, and the superior colliculus (SC), which projects to the pulvinar among other structures. These two pathways are often referred to as the primary and the secondary pathway respectively and are inter-connected, notably at the level of the SC. Importantly, visual inputs in the SC converge onto one morphologically and molecularly defined cell type: the wide-field vertical cells (WFV). WFV cells dendritic arborizations sample a large part of the visual field and we have recently shown that these cells are modulated by locomotion and receive inputs from multiple brain region. Their morphology and projection to the pulvinar are conserved across species. These observations place WFV cells as key integrators of visual and non-visual information. To understand how WFV cells integrate information, the investigator will perform causal experiments in which different sources of inputs will be manipulated in mice (Mus musculus) while the activity of WFV cells will be assessed using calcium imaging. This will be followed by the identification of the connectivity of the WFV in another animal model, the tree shrew (Tupaia belangerii), which is highly visual and closely related to primates. This will address the conservation of the brain-wide circuit across species, thus opening new alleys for translational work. To resolve existing discrepancy concerning the overall visual responses in the SC, the visual responses of WFV cells will be characterized in tree shrews. This particular cell-type will be isolated using an optogenetic approach and electrophysiological recordings, thus allowing for the characterization of visual response preference of these cells. This project will yield compelling results regarding the integration of visual information across the visual system, allow a direct comparison of the same defined cell-type across two species, and reveal how internal states shape visual responses in the SC.

项目摘要（请参阅说明）： 并行提取独特的视觉特征，以稍后沿视觉进行调节 途径。可以尽早发现两种平行途径，而视网膜的预测针对 背侧侧向核的核，该核针对皮质区域，而上丘（SC）（SC） 在其他结构和其他结构中进行项目。这两个途径通常被称为主要 次级途径分别是相互连接的，特别是在SC的水平上。 重要的是，SC中的视觉输入在形态和分子定义的细胞类型上收敛到一个： 广场垂直细胞（WFV）。 WFV细胞树突状作用样品样品的大部分视野 我们最近表明，这些单元是通过运动调节的，并从多个接收输入 大脑区域。它们的形态和对普尔维纳尔的投影在各种物种之间是保守的。这些 观察结果将WFV细胞作为视觉和非视觉信息的关键集成因子。 为了了解WFV单元如何整合信息，研究人员将在 在小鼠（mus musculus）中，哪些输入来源将被操纵，而WFV细胞的活性 将使用钙成像进行评估。随后将识别 WFV在另一个动物模型中，树sh（tupaia belangerii），它高度视觉且密切相关 灵长类动物。这将解决跨物种的脑范围电路的保护，从而打开新的 小巷翻译工作。解决有关现有的差异 SC，WFV细胞的视觉响应将在树生中表征。这种特殊的细胞类型将是 使用光遗传学方法和电生理记录进行分离，从而允许 这些细胞的视觉反应偏好表征。该项目将产生令人信服的结果 关于整个视觉系统的视觉信息的集成，允许直接比较 在两个物种中相同定义的细胞类型，并揭示内部状态如何塑造视觉反应 sc。