Advanced Microarray Technology for Pathogen Surveillance

用于病原体监测的先进微阵列技术

• 批准号: 7476524
• 负责人: KATHY L ROWLEN
• 金额: $ 48.7万
• 依托单位: INDEVR, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7476524
• 关键词: Academia; Aldehydes; Applied Research; Award; Binding; Biological; Biological Warfare; Businesses; Centers for Disease Control and Prevention (U.S.); Chemistry; Colorado; Commercial Sectors; Country; DNA; Data Collection; Detection; Development; Diagnosis; Diagnostic; Disease; Drug Formulations; Engineering; Event; Eye; Film; Fluorescence; Genetic; Glass; Goals; Government; Grant; Hand; Hour; Human; Influenza; Institution; Lectin; Localized; Medical Surveillance; Methodology; Methods; Microarray Analysis; Monitor; National Institute of Allergy and Infectious Disease; Nature; Nucleic Acids; Patients; Phase; Polymers; Population; Protein Microchips; Proteins; Protocols documentation; Public Health; Range; Reader; Reagent; Research; Role; Sampling; Screening procedure; Sialic Acids; Signal Transduction; Silicon; Small Business Technology Transfer Research; Solid; Surface; System; Techniques; Technology; Testing; Time; Universities; Vaccine Production; Viral; Virus; Vision; Visual; Work; World Health Organization; aptamer; base; biodefense; cost; day; design; improved; influenzavirus; innovation; light weight; new technology; novel; pathogen; photopolymerization; polymerization; portability; programs; prototype; rapid detection; response; viral RNA

DESCRIPTION (provided by applicant): This proposal is in response to NIAID's call for Cooperative Research Partnerships for Biodefense (RFAAI-05-019). Consistent with the stated objectives of this RFA, our established team of chemists, biologists and engineers from the commercial sector, academia and government, proposes to conduct the research necessary to advance three potential products that are anticipated to significantly enhance the utility of microarrays for pathogen surveillance and biodefense. Influenza surveillance will be targeted as the first specific application of the technology. Because human-adapted influenza virus is a readily transmitted, primarily airborne pathogen, and because the potential exists for the virus to be genetically engineered into novel forms, influenza A represents a serious public health and biodefense concern. The Centers for Disease Control and Prevention and the World Health Organization have strongly emphasized the importance of global influenza surveillance because of its critical role in determination of viral strain identification (required for vaccine production) and disease distribution. While there are 112 institutions in 83 countries that serve as National Influenza Centers for surveillance, coverage of the world's population remains quite limited, and significant efforts are needed to enhance influenza surveillance. A major factor in this dearth of coverage is the current expensive and labor-intensive (days) method for strain analysis. In order to realize the tremendous potential of the microarray platform for rapid disease diagnosis and pathogen identification, it is essential that alternative, inexpensive and easy-to-use detection technologies be developed. Use of microarrays as effective monitoring systems in biodefense also requires field portability for prompt and widespread deployment. Our vision is a diagnostic kit that would enable field application of microarrays with an analysis time of hours instead of days and at a cost consistent with the need for world-wide distribution. The kit would provide the means for rapid target extraction from the sample, on-chip signal amplification resulting in a polymer detectable by eye, and a hand-held reader to be used for interpretation of multi-pathogen microarrays. The proposed Specific Aims are to develop the following three products: a) a simple sample handling kit for virus pre-concentration, b) an innovative non-enzymatic technology for amplifying signals from bio-recognition events (both nucleic acids and proteins) directly on microarrays, and c) a robust, inexpensive field-portable microarray reader.

描述（由申请人提供）：该提案是针对Niaid呼吁生物实施合作研究合作伙伴的呼吁（RFAAI-05-019）。与该RFA的既定目标相一致，我们成熟的化学家，生物学家和工程师团队，来自商业部门，学术界和政府，提议进行必要的研究，以推动三种潜在的产品，这些产品预计可显着增强微阵列对病原体监测和生物效果的实用性。流感监视将作为该技术的第一个特定应用。由于人类适应的流感病毒是一种容易传播的，主要是空降病原体，并且由于存在将病毒遗传化为新形式的潜力，因此流感是一种严重的公共卫生和生物反应的关注。疾病控制和预防中心和世界卫生组织强烈强调了全球流感监测的重要性，因为它在确定病毒株鉴定（疫苗生产所需）和疾病分布中的重要作用。虽然83个国家 /地区有112个机构作为国家流感中心的监视中心，但对世界人口的覆盖范围仍然非常有限，并且需要巨大的努力来增强流感的监视。缺乏覆盖范围的一个主要因素是当前昂贵且劳动密集型（天）的应变分析方法。为了实现对快速疾病诊断和病原体鉴定的微阵列平台的巨大潜力，必须开发替代，廉价且易于使用的检测技术至关重要。在BiodeFense中，将微阵列用作有效的监视系统也需要现场便携性，以便迅速和广泛部署。我们的愿景是一个诊断套件，它可以通过数小时而不是几天的分析时间来实现微阵列的现场应用，并且以符合全球分布的需求一致。该套件将提供从样品中快速靶向提取的方法，片上信号放大，从而导致可通过眼睛检测到的聚合物，以及用于解释多病原体微阵列的手持式读取器。提出的具体目的是开发以下三种产品：a）一种简单的样品处理套件，用于病毒前浓缩，b）一种创新的非酶技术，用于从生物识别事件（核酸和蛋白质都）放大信号（既有微阵列），以及直接在微阵列上，以及C）在稳健，廉价的现场模拟体育体验中质量较高的现场模拟体育奖。