Democratizing embryo biomechanics: Development of robust and accessible methods to quantify sub-cellular mechanics in vivo

胚胎生物力学民主化：开发稳健且易于使用的方法来量化体内亚细胞力学

基本信息

批准号：
10724783
负责人：
Jose R Alvarado
金额：
$ 43.59万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-02 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10724783
关键词：
Actomyosin Address Animals Atomic Force Microscopy Benchmarking Biological Biomechanics Cell membrane Cells Cellular biology Communities Computers and Advanced Instrumentation Congenital Abnormality Cytoplasm Data Defect Development Embryo Embryonic Development Engineering Goals Heterogeneity Human Intercellular Junctions Label Lasers Link Magnetism Mammals Measurement Measures Mechanics Membrane Methods Microdissection Microscope Molecular Morphogenesis Motion Nature Oranges Organ Pattern Phase Physics Physiologic pulse Property Protein Dynamics Proteins Reporter Research Personnel Resolution Rheology Sampling System Technology Time Tissues Twin Multiple Birth Viscosity Work biomechanical test cell behavior cell cortex cell motility cell type convergent extension driving behavior embryo tissue ferrofluid fluorescence imaging improved in vivo innovation mechanical force mechanical properties novel particle planar cell polarity success temporal measurement tool vertebrate embryos

项目摘要

Abstract: It is now widely recognized that any comprehensive understanding of morphogenesis will require careful quantification of mechanical forces in vivo. However, twin challenges stand in our way: First, we currently possess only a sparse sampling of subcellular mechanics in embryos, in part because such analyses are limited by the need for advanced instrumentation. Second, most analyses of mechanical properties do not provide sufficient spatial resolution to quantify localized mechanical heterogeneities in discrete sub-regions of the cell. Here, we attack both problems by developing robust methods for non-invasive quantification of the mechanical properties of both cell-cell junctions and the cytoplasm. The proposal will develop these tools in the context of vertebrate embryo axis elongation, but both methods should be widely accessible, requiring only fluorescent reporters and standard microscopes. Success of the project would 1) improve our depth of understanding through their high spatial and time resolution and their non- invasive nature and 2) improve our breadth of understanding by making quantitative biomechanical analysis in embryos far more accessible to a wide range of investigators.

抽象的：现在已广泛认识到，对形态发生的任何全面理解都将需要仔细定量体内的机械力。但是，双重挑战在我们的方式：首先，我们目前只有少量的亚细胞力学在胚胎中，在部分是因为此类分析受到高级仪器的需求的限制。第二，机械性能的大多数分析都不提供足够的空间分辨率来量化细胞离散子区域中的局部机械异质性。在这里，我们攻击两个通过开发强大的方法来进行机械定量的鲁棒方法来解决问题细胞 - 细胞连接和细胞质的性质。该提案将在脊椎动物胚胎伸长的上下文，但两种方法都应广泛访问，仅需要荧光记者和标准显微镜。该项目的成功将1）通过他们的高空间和时间分辨率以及非 - 侵入性的性质和2）通过进行定量来提高我们的理解广度胚胎中的生物力学分析对于广泛的研究者更容易获得。