Role of iron homeostasis in cranberry's bio-effects

铁稳态在蔓越莓生物效应中的作用

• 批准号: 7196590
• 负责人: Maolin Guo
• 金额: $ 21.91万
• 依托单位: UNIVERSITY OF MASSACHUSETTS DARTMOUTH
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7196590
• 关键词: Adhesions; Affinity; Aging; American; Anti-Bacterial Agents; Antioxidants; Antiviral Agents; Attention; Bacterial Infections; Beverages; Binding; Biochemical; Biochemistry; Biological; Cardiovascular Diseases; Chemical Structure; Chemistry; Complex; Cranberries; Development; Electrochemistry; Escherichia coli; Fruit; Gastric ulcer; Goals; Health; Health Benefit; Homeostasis; Human; Infection; Iron; Iron Chelation; Juice; Knowledge; Medicine; Methods; Microbial Biofilms; Microbiology; Molecular Biology; Molecular Mechanisms of Action; Powder dose form; Prevention; Property; Research; Role; Stroke; Structure; Techniques; Testing; Tooth Diseases; Urinary tract infection; Vaccinium macrocarpon; Work; X-Ray Crystallography; anticancer activity; base; design; extracellular; novel; nutrition; pathogenic bacteria; research study

DESCRIPTION (provided by applicant): Cranberry and its related fruits and their beverage products have received considerable attention for their health beneficial effects to human beings. These health benefits include prevention and treatment of urinary tract infections (UTIs), stomach ulcers, gum diseases, dental infections, blocking biofilm formation associated with bacterial infections, providing protection against aging, stroke, cardiovascular diseases and more recently anticancer activity. In spite of research in the past few decades, little is known about the molecular mechanism of action of these health beneficial effects. We have initiated research exploring the possible mechanisms. Based on the chemical structures and bioactivities of certain cranberry constituents, as well as the current understanding of iron homeostasis and its relation to medicine, a novel molecular mechanism of action for certain health benefits of cranberry has been hypothesized in this proposal. This mechanism may give reasonable biochemical explanations to many of the health benefits demonstrated for cranberry and its related fruits so far. The general goal of the work proposed here is to test this hypothesis. Multi-disciplinary approach including using knowledge and techniques from chemistry, electrochemistry, biochemistry and molecular biology, microbiology, spectroscopic methods and X-ray crystallography have been designed to test the proposed mechanisms. Experiments described herein are designed to elucidate the iron-binding properties of certain cranberry constituents, including structures and affinities, and the effects of these properties on the antibacterial, antioxidant and antitumor properties of cranberry and its related products. This research has profound impact to applying cranberry and its related fruits and products in human health, nutrition and medicine.

描述（由申请人提供）：蔓越莓及其相关水果及其饮料产品对人类的健康有益影响受到了极大的关注。这些健康益处包括预防和治疗尿路感染（UTI），胃溃疡，牙龈疾病，牙齿感染，阻断与细菌感染相关的生物膜形成，提供针对衰老，中风，心血管疾病的保护，以及最近的抗癌活性。尽管在过去的几十年中进行了研究，但对这些健康有益效应的作用的分子机理知之甚少。我们启动了研究可能机制的研究。基于某些蔓越莓成分的化学结构和生物活性，以及​​当前对铁稳态的理解及其与医学的关系，在本提案中已经提出了一种新型的蔓越莓健康作用机理，以实现某些健康益处。这种机制可能会为迄今为止蔓越莓及其相关水果所证明的许多健康益处提供合理的生化解释。这里提出的工作的一般目标是检验这一假设。多学科方法包括使用化学，电化学，生物化学和分子生物学，微生物学，光谱方法和X射线晶体学的知识和技术，以测试提出的机制。本文所描述的实验旨在阐明某些蔓越莓成分的铁结合特性，包括结构和亲和力，以及这些特性对蔓越莓及其相关产物的抗菌，抗氧化剂和抗肿瘤特性的影响。这项研究对将蔓越莓及其相关的水果和产品应用于人类健康，营养和医学具有深远的影响。