Semiconducting Polymer Dots for Multiplexed Assays

用于多重检测的半导体聚合物点

• 批准号: 9141866
• 负责人: Jiangbo Yu
• 金额: $ 49.04万
• 依托单位: LAMPROGEN, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9141866
• 关键词: Adoption; Antigens; Area; Benchmarking; Biological Assay; Biological Sciences; Biomedical Research; Caliber; Cellular biology; Clinical; Communities; Complement 2; Detection; Development; Diagnostic; Dyes; Exhibits; Financial compensation; Flow Cytometry; Fluorescence; Fluorescent Dyes; Fluorescent Probes; Goals; Image; Lasers; Marketing; Methods; Performance; Phase; Phycoerythrin; Play; Polymers; Production; Property; Protocols documentation; Quality Control; Reagent; Reproducibility; Resolution; Role; Series; Small Business Innovation Research Grant; Surface; Techniques; Time; Translating; Viola; Width; Work; base; community science; density; design; diagnostic assay; fluorophore; functional group; genetic analysis; manufacturing process; nanoparticle; optical spectra; particle; public health relevance; success; technique development; zeta potential

 DESCRIPTION (provided by applicant): Fluorescence based methods play an essential role in modern cell biology and biomedical research. Further development of these techniques has been hindered by the lack of suitable fluorescent probes. To overcome this challenge, various fluorescent nanoparticles have been developed. Among those, semiconducting polymer dots (Pdots) exhibit superior properties such as high brightness, fast emission rate, and excellent photo stability. Fluorescence based techniques encompass a broad area with a large market size, ranging from immuno-assays to super-resolution imaging. And while Pdot has proven to be an excellent general fluorescent probe, to maintain focus, to penetrate the market, and to generate adoption by the biomedical community, we have decided to go after flow cytometry applications, owing to its large market potential. During our 6-month Phase I SBIR project, we have developed Pdot450 and Pdot515 with ultra- high brightness, narrow-band emission, and high photo stability. To achieve this goal of penetrating the flow cytometry market, this Phase II SBIR project proposes to develop 5 additional Pdot probes to complement the 2 developed during Phase I. After this Phase II project, we will have completed a set of 7 Pdots so we may launch a Pdot series with high brightness and narrow emissions for flow cytometry applications. Furthermore, we will establish pre-production and quality control protocols for manufacturing this Pdot series.

 描述（由申请人提供）：基于荧光的方法在现代细胞生物学和生物医学研究中发挥着重要作用。由于缺乏合适的荧光探针，这些技术的进一步发展受到阻碍。为了克服这一挑战，已经开发了各种荧光纳米颗粒。其中，半导体聚合物点（Pdot）表现出优异的性能，例如高亮度、快速发射速率和出色的光稳定性，其基于荧光的技术涵盖了广阔的领域和巨大的市场规模。虽然 Pdot 已被证明是一种出色的通用荧光探针，但为了保持焦点、渗透市场并获得生物医学界的采用，我们决定追求流式细胞术应用，由于其巨大的市场潜力，我们在为期6个月的第一期SBIR项目中，开发了具有超高亮度、窄带发射和高光稳定性的Pdot450和Pdot515来实现这一目标。在流式细胞仪市场，第二阶段 SBIR 项目建议开发 5 个额外的 Pdot 探针，以补充第一阶段开发的 2 个 Pdot 探针。在第二阶段项目之后，我们将完成一组 7 个 Pdot，因此我们可能会推出具有高性能的 Pdot 系列。此外，我们将为制造该 Pdot 系列建立预生产和质量控制协议。