Next Generation Sequencing Core

下一代测序核心

• 批准号: 10714179
• 负责人: ERLE S. ROBERTSON
• 金额: $ 45.67万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10714179
• 关键词: ATAC-seq; Address; Award; Bioinformatics; Biological Assay; Biometry; Cancer Center; Center Core Grants; ChIP-seq; Complement; Complex; Core Facility; DNA Sequencing Facility; DNA sequencing; Data; Data Analyses; Genetic Transcription; Genomics; Goals; Herpesviridae; High-Throughput Nucleotide Sequencing; Human Genome; Human Herpesvirus 4; Human Herpesvirus 8; Hypoxia; Leadership; Libraries; Maps; Methodology; Methods; Oncogenic; Oncogenic Viruses; Online Systems; Preparation; Procedures; Protocols documentation; Research Personnel; Sampling; Services; Standardization; Technical Expertise; Technology; Work; chromatin immunoprecipitation; data management; experience; experimental study; follow-up; medical schools; member; next generation sequencing; programs; success; transcriptome sequencing; ATAC-seq; Address; Award; Bioinformatics; Biological Assay; Biometry; Cancer Center; Center Core Grants; ChIP-seq; Complement; Complex; Core Facility; DNA Sequencing Facility; DNA sequencing; Data; Data Analyses; Genetic Transcription; Genomics; Goals; Herpesviridae; High-Throughput Nucleotide Sequencing; Human Genome; Human Herpesvirus 4; Human Herpesvirus 8; Hypoxia; Leadership; Libraries; Maps; Methodology; Methods; Oncogenic; Oncogenic Viruses; Online Systems; Preparation; Procedures; Protocols documentation; Research Personnel; Sampling; Services; Standardization; Technical Expertise; Technology; Work; chromatin immunoprecipitation; data management; experience; experimental study; follow-up; medical schools; member; next generation sequencing; programs; success; transcriptome sequencing

Summary Core C: Next Generation Sequencing core (NGSC) The next generation sequencing core (NGSC) will be responsible for all next generation sequencing activities by the four projects. All sequencing activities will be performed in Core C. RNA-Seq, ChIP-Seq, ATAC-Seq, Core C will be responsible for 1) performing NGS-assays; 2) generating library for Illumina next-generation sequencing, 3) mapping sequence data to EBV, KSHV, MPyV and human genomes, and 4) providing all follow-up bioinformatics and biostatistics analyses for the overall program. These goals will be accomplished in conjunction with the Penn Genomics, Next-Gen-Sequencing and Bioinformatics Cores, which provides Illumina Next Generation Sequencing as a core service. Final data management and analyses will be made accessible to each project investigator via a password protected shared web-based browser for program investigators. Expertise in all aspects of Next-Gen-Sequencing (NGS), Chromatin immunoprecipitation, and Bioinformatics support will be provided to the projects for the lifetime of the award. The NGS core will be led by experience leaders in administration and technical expertise to ensure that the core will be successful. Dr. Lieberman, is an expert in NGS and is familiar with all aspects of NGS technologies. Dr. Robertson has led many large complex programs, and is familiar with management and coordination of the technical expertise needed for success. Thus, the leadership is familiar with different NGS methodologies and bioinformatics analyses as applied to oncogenic herpesviruses and the human genome. Core C leaders have both provide unique expertise that complement each other. The NGS Core C will facilitate the ability of all members of the Program to standardize methods for sample preparation and for post-sequence bioinformatics analyses. The work will be performed at the Perelman School of Medicine in space adjacent to the Robertson lab, and in conjunction with the Penn Genomics NGS Core, and Bioinformatics core supported in part by the Abramson Cancer Center.

概括 核心C：下一代测序核心（NGSC） 下一代测序核心（NGSC）将负责所有下一代测序活动 由四个项目。所有测序活动都将在Core C. RNA-Seq，Chip-Seq，Atac-Seq， 核心C将负责1）执行NGS-says； 2）生成Illumina下一代的图书馆 测序，3）将序列数据映射到EBV，KSHV，MPYV和人类基因组，以及4） 整体计划的后续生物信息学和生物统计学分析。这些目标将在 与Penn Genomics，下一代和生物信息学核心的结合，提供Illumina 下一代排序作为核心服务。最终的数据管理和分析将被访问 通过密码保护的每个项目调查员为程序调查人员提供密码保护的共享基于网络的浏览器。 在下一代序列（NGS），染色质免疫沉淀和生物信息学方面的各个方面的专业知识 奖励一生的项目将提供支持。 NGS核心将由经验领导 行政和技术专长领域的领导者，以确保核心将取得成功。 Lieberman博士是 NGS专家，熟悉NGS技术的各个方面。罗伯逊博士领导了许多大型 复杂的计划，并且熟悉管理和协调所需的技术专长 成功。因此，领导层熟悉不同的NGS方法论和生物信息学分析 应用于致癌疱疹病毒和人类基因组。 C核心领导者都提供独特的 相互补充的专业知识。 NGS核心C将促进该计划的所有成员的能力 标准化样品制备和序列生物信息学分析的方法。工作将 可以在罗伯逊实验室附近的佩雷尔曼医学学院和 与Penn Genomics NGS核心以及Abramson Cancer支持的生物信息学核心有关 中心。