Osteogenic Calcium Phosphate Nanoparticles with Designable Drug Release Kinetics

具有可设计药物释放动力学的成骨磷酸钙纳米颗粒

• 批准号: 9096064
• 负责人: Vuk Uskokovic
• 金额: $ 3.19万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-05 至 2016-10-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9096064
• 关键词: Address; Antibiotics; Antifungal Agents; Area; Award; Biocompatible Materials; Biodegradation; Biological; Biomaterials Research; Biomedical Engineering; Cell Culture Techniques; Chemicals; Clindamycin; Complement; Consult; Defect; Dental; Dental Schools; Drug Compounding; Drug Controls; Drug Delivery Systems; Drug usage; Emulsions; Encapsulated; Engineering; Fibroblasts; Filler; Fluorescent Dyes; Funding; Goals; Grant; Growth; Health; Hemostatic Agents; Image; In Vitro; Institutes; Intravenous; K-Series Research Career Programs; Kinetics; Knowledge; Lead; Marshal; Mentors; Methods; Morphology; Natural regeneration; Oral; Osteoblasts; Osteomyelitis; Particle Size; Pathway interactions; Performance; Pharmaceutical Preparations; Phase; Polymers; Precipitation; Procedures; Property; Protocols documentation; Research; Research Activity; Research Training; Strategic Planning; Suspension substance; Suspensions; Testing; Therapeutic; Time; Tissues; Training; Training Activity; Training Programs; Trauma; United States National Institutes of Health; Work; Writing; antimicrobial; base; bone; calcium phosphate; craniofacial; design; drug production; implantation; materials science; nanoparticle; nanosized; nanostructured; osteogenic; particle; response; skills; stoichiometry; theranostics

This K99/R00 Mentored Research Career Development Award application is being submitted to the National Institute on Dental and Craniofacial Research (NIDCR). The candidate, Dr. Vuk Uskokovic, is a physical chemist and nanotechnologist who seeks to obtain additional training in the area of cell culture experimentation and other biological methods of analysis through this award. His aim is to develop research skills in the area of multifunctional and nanostructured biomaterials for reparation of hard tissues. The goals of this proposal would enhance Dr. Uskokovic's knowledge of fabrication of smart biomaterials for simultaneous time-controlled drug release and regeneration of diseased hard tissues. Proposed is the synthesis of calcium phosphate/polymer composite particles that encapsulate clinmadycin, a drug used in the treatment of osteomyelitis, as well as testing of the antimicrobial properties and osteinductive performance of the given material in vitro. The central hypothesis of the proposed study is that stoichiometry and the particle size of calcium phosphates can be used to tune the kinetics of the release of the encapsulated drug. Dr. Uskokovic will conduct his training and research activities under the guidance of an expert mentoring team that includes Drs. Tejal Desai, Grayson Marshall, Stefan Habelitz, Wu Li, and Peter Loomer. He will receive additional consulting from Drs. Mauro Ferrari, Antoni Tomsia, and Charles Hoover. Given that bioengineering and biomaterials research approaches to regeneration of oral and craniofacial tissues has been selected as one of the prioritized goals by the NIDCR (Objective I-5, Goal 1, NIDCR Strategic Plan 2009-2013), the goals of the current application will address highly actual oral, dental and craniofacial health problems. Training Dr. Uskokovic through this Pathway to Independence Award on top of his expertise in materials science and engineering and support by the UCSF Dental School, the most funded one by the NIH in the past 15 years, also complies with all three objectives of Goal 2 of the NIDCR Strategic Plan. Dr. Uskokovic will conduct a study which will involve: a) Synthesis of multifunctional therapeutic and osteoinductive calcium phosphate nanoparticles by means of co-precipitation from ultrasonically agitated emulsions; b) Drug release studies in bacterial and cell cultures. With respect to the proposed hypothesis, the central aim of this study is to derive fundamental correlations between the dissolution rate and drug delivery efficiency of calcium phosphate based carriers and their chemical composition and microstructure. This K99/R00 award is conceived to lead to an R01 grant, the writing of which will begin before the end of the award period.

此 K99/R00 指导研究职业发展奖申请将提交至 国家牙科和颅面研究所 (NIDCR)。候选人 Vuk 博士 Uskokovic 是一位物理化学家和纳米技术专家，他寻求获得以下领域的额外培训： 细胞培养实验和其他生物分析方法领域 奖。他的目标是发展多功能和纳米结构领域的研究技能 用于修复硬组织的生物材料。该提案的目标将增强博士的能力。 乌斯科科维奇在同时时间控制的智能生物材料制造方面的知识 药物释放和患病硬组织的再生。提出的是钙的合成 封装克林霉素的磷酸盐/聚合物复合颗粒，克林霉素是一种用于 骨髓炎的治疗以及抗菌特性和骨诱导的测试 给定材料的体外性能。拟议研究的中心假设是 磷酸钙的化学计量和粒径可用于调节反应动力学 包封药物的释放。乌斯科科维奇博士将开展他的培训和研究活动 在包括博士在内的专家指导团队的指导下。特加尔·德赛、格雷森 马歇尔、斯特凡·哈贝利茨、吴力和彼得·卢默。他将接受来自以下方面的额外咨询： 博士。毛罗·法拉利、安东尼·汤姆西亚和查尔斯·胡佛。鉴于生物工程和 口腔和颅面组织再生的生物材料研究方法 被 NIDCR 选为优先目标之一（目标 I-5、目标 1、NIDCR 战略 计划 2009-2013），当前应用程序的目标将解决高度实际的口腔、牙科和 颅面健康问题。通过这条独立之路训练乌斯科科维奇博士 因其在材料科学与工程方面的专业知识以及加州大学旧金山分校的支持而获奖 牙科学院是过去 15 年来 NIH 资助最多的学院，也符合所有三个标准 NIDCR 战略计划目标 2 的目标。乌斯科科维奇博士将进行一项研究，该研究将 涉及：a) 多功能治疗和骨诱导磷酸钙的合成 通过超声波搅拌乳液共沉淀形成纳米颗粒； b) 药物 细菌和细胞培养物中的释放研究。对于所提出的假设， 本研究的中心目的是得出溶出率和溶出率之间的基本相关性 磷酸钙基载体的药物递送效率及其化学成分和 微观结构。该 K99/R00 奖项旨在获得 R01 资助，其写作内容 将在奖励期结束前开始。