Bone Lead Standardization Program

骨铅标准化计划

• 批准号: 6784648
• 负责人: Patrick J. Parsons
• 金额: $ 33.79万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6784648
• 关键词: analytical method; atomic absorption spectrometry; bone; chemical registry /resource; chemical standardization; diagnosis quality /standard; goats; lead; lead poisoning; mass spectrometry; measurement; method development; radiofluorescent probe; statistics /biometry

DESCRIPTION (provided by applicant): The aim of this proposal is to create a Standardization Program for Bone Lead measurements (BLSP) obtained via reference methods and via in vivo X-Ray Fluorescence (XRF). The BLSP will fulfill current needs of the bone lead methodologies: lack of appropriate certified reference materials (RMs) and lack of traceability. The BLSP will also provide RMs for electrothermal atomic absorption spectrometry (AAS) and inductively coupled plasma-mass spectrometry (ICP-MS) and will conduct an interlaboratory validation study among laboratories using those methods. The program will also provide RMs for XRF, thereby providing traceability to SI units, and will conduct an interlaboratory validation study among laboratories using XRF for in vivo bone lead measurements. There are six goals: first to cross-validate AAS and ICP-MS in the Wadsworth Trace Elements Laboratory of the New York State Department of Health (NYS DOH); second to establish a national repository of RMs for bone lead measurement via AAS and ICP-MS traceable to NIST SRM 1486 Bone Meal and 1400 Bone Ash; third, to conduct an interlaboratory validation of AAS and/or ICP-MS for bone lead measurement. The fourth goal is to assemble a national repository of RMs for bone lead XRF by preparing two sets of well-characterized goat bone samples containing endogenous lead concentrations that encompass those found in human populations; the fifth goal is to conduct an in vitro XRF interlaboratory validation and comparison study using the two sets of goat bone samples. The first set of XRF RMs will be destroyed as part of the process of obtaining reference concentrations via AAS and ICP-MS for both surface and core bone that are traceable to SI units. The reference concentrations thus obtained will form the basis of the assessment of XRF accuracy. The second set of XRF RMs will be created and used for a second interlaboratory comparison and will be preserved for long-term use. The sixth and last goal is to conduct an in vivo XRF validation study using the euthanized members of the NYS DOH goat colony that will provide the RMs. XRF-measured in vivo concentrations will be compared to the reference concentrations obtained from AAS and ICP-MS.

描述（由申请人提供）：该提案的目的是创建用于通过参考方法获得的骨头铅测量（BLSP）的标准化程序，并通过体内X射线荧光（XRF）获得。 BLSP将满足骨骼铅方法的当前需求：缺乏合适的认证参考材料（RMS）和缺乏可追溯性。 BLSP还将提供电热原子吸收光谱法（AAS）的RMS，并提供电感耦合的血浆质量光谱法（ICP-MS），并将使用这些方法对实验室进行实验室间验证研究。该程序还将为XRF提供RMS，从而为SI单元提供可追溯性，并将使用XRF进行体内骨头铅测量的实验室进行实验室间验证研究。有六个目标：首先在纽约州卫生部（NYS DOH）的Wadsworth Trace Elements实验室中对AAS和ICP-MS进行交叉估算。其次是通过AAS和ICP-MS建立用于骨头铅测量的全国RMS存储库，可追溯到NIST SRM 1486骨粉和1400骨灰；第三，用于对AAS和/或ICP-MS进行实验室间验证，以进行骨骼铅测量。第四个目标是通过准备两组含有内源性铅浓度的特征良好的山羊骨样品来组装骨骼铅XRF的国家存储库，这些山羊骨样品包括内源性铅浓度，这些铅含量涵盖了在人类种群中发现的。第五个目标是使用两组山羊骨样品进行体外XRF实验室间验证和比较研究。第一组XRF RMS将被破坏，作为通过AAS和ICP-MS获得参考浓度的一部分，用于表面和核心骨，可追溯到SI单元。因此获得的参考浓度将构成评估XRF准确性的基础。第二组XRF RMS将被创建并用于第二个实验室间比较，并将保存供长期使用。第六个也是最后一个目标是使用将提供RMS的NYS DOH山羊殖民地的安乐死成员进行体内XRF验证研究。将体内浓度测量的XRF测量与从AAS和ICP-MS获得的参考浓度进行比较。