Cancer Center Tumor Phenotyping Core

癌症中心肿瘤表型核心

• 批准号: 6729916
• 负责人: SUE C HEFFELFINGER
• 金额: $ 25.05万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6729916
• 关键词: biomedical facility; chromosome translocation; fluorescence microscopy; fluorescent in situ hybridization; immunocytochemistry; neoplasm /cancer genetics; nucleic acid amplification techniques; nucleic acid probes; nucleic acid purification; nucleic acid quantitation /detection; nucleic acid sequence; phenotype; polymerase chain reaction

DESCRIPTION (provided by applicant): In 1996, Ohio's death rate from all cancers was 13th highest among the 50 states and the District of Columbia. Ohio ranked 16th highest for death rates from lung cancer, 12th from colorectal cancer and 6th for breast cancer deaths. The area in and around Cincinnati is in the top 10th percentile of mortality from cancer for Blacks, Whites, males and females. Hamilton County (site of the University of Cincinnati) has a statistically significant higher cancer incidence than Ohio overall. These data have ignited clinicians, researchers, and administrators to develop collaborative programs that will spark new investigations into the basic biology of cancer and foster programs to allow translation of this new knowledge into clinical care. This proposal is one of four that are the direct result of a series of discussion groups, including clinicians and basic researchers, to develop program strategies to meet these clinical needs. Both clinical/translational and basic researchers felt there was a paucity of institutional resources for tissue-based research and that a Tumor Phenotyping Core would significantly enhance the research capacity in the College of Medicine. This core will work closely with two of the other proposed cores, which provide (1) genomics/proteomics and (2) routine histology, preparation of tissue microarrays, and laser capture microdissection. The Tumor Phenotyping Core will provide resources for automated immunohistochemistry, in situ hybridization, fluorescence in situ hybridization (FISH), RNA and DNA isolation, fluorescence microscopy, and real time PCR. This core will provide resources to the 30 currently NCI funded investigators in the institution, as well as providing seed grant monies for start up projects to encourage additional cancer related research in the institution. Resources will be utilized based upon review of proposals by an Internal Advisory Committee and will provide personnel as well as 50% of supply costs for approved projects. This core is an expansion of a current facility in the Department of Pathology that serves clinical and research needs in the institution. Expansion of personnel and availability of supply dollars will allow this core to serve numerous investigators in the cancer community.

描述（由申请人提供）： 1996年，俄亥俄州所有癌症的死亡率在50个癌症中排名第13 各州和哥伦比亚特区。 俄亥俄州的死亡率排名第16 来自肺癌，结直肠癌的第十二，乳腺癌第六 死亡人数。辛辛那提及其周围地区处于最高的10个百分位数 黑人，白人，男性和女性的癌症死亡率。汉密尔顿县 （辛辛那提大学的地点）具有更高的统计学意义 总体上比俄亥俄州的癌症发生率。这些数据点燃了临床医生， 研究人员和管理人员开发协作计划 引发对癌症和寄养计划的基本生物学的新调查 允许将这种新知识转化为临床护理。这个建议 是四个是一系列讨论组的直接结果之一， 包括临床医生和基础研究人员，制定计划策略 满足这些临床需求。临床/转化和基础研究人员 认为基于组织的研究缺乏机构资源 并且肿瘤表型核心将显着增强研究 医学院的能力。该核心将与两个 其他提出的核心，提供（1）基因组/蛋白质组学和（2） 常规组织学，组织微阵列的制备和激光捕获 显微解剖。肿瘤表型核心将为 自动免疫组织化学，原位杂交，原位荧光 杂交（FISH），RNA和DNA分离，荧光显微镜以及真实 时间pcr。该核心将为当前的NCI资助的30个资源提供资源 该机构的调查人员以及为种子赠款款项 启动项目，以鼓励其他与癌症相关的研究 机构。资源将根据对建议的审查来利用 内部咨询委员会，将提供人员以及50％ 批准项目的供应成本。该核心是电流的扩展 病理学系的设施为临床和研究服务 机构的需求。人员的扩大和供应可用性 美元将使该核心能够为癌症的众多调查员提供服务 社区。