Multiphoton microscope replacement for USC shared resource

多光子显微镜替代南加州大学共享资源

• 批准号: 9274881
• 负责人: JANOS PETI-PETERDI
• 金额: $ 59.98万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274881
• 关键词: Animals; Biological Markers; Biomedical Engineering; Biomedical Research; Brain; Cost Sharing; Degenerative Disorder; Dental; Devices; Disease; Equipment; Funding; Gerontology; Health; Housing; Human; Image; Institutes; Kidney; Lacrimal gland structure; Lasers; Liver Stem Cell; Lung; Maintenance; Malignant neoplasm of brain; Manufacturer Name; Microscope; Microscopy; Modeling; Organ; Pharmacologic Substance; Pharmacy Schools; Regenerative Medicine; Research; Research Activity; Research Personnel; Research Support; Resource Sharing; School Dentistry; Schools; Science; Services; Stem cells; System; Technology; Testing; Tissue imaging; Tissues; United States National Institutes of Health; base; cellular imaging; imaging modality; imaging study; improved; in vivo; instrumentation; intravital imaging; medical schools; multi-photon; neurogenetics; novel; operation; quantitative imaging; repaired; temporal measurement

MULTIPHOTON MICROSCOPE REPLACEMENT FOR USC SHARED RESOURCE This proposal is submitted to purchase a new, high-end Leica SP8 AOBS multiphoton microscope with 1300nm IR laser for the Multi-Photon Microscopy Core at the Keck School of Medicine at USC. The new microscope will replace an old, non-operational model which is beyond repair and no longer serviced by the manufacturer. The new microscope will be a unique system dedicated to provide ultra-sensitive, deep tissue imaging of intact living organs in vivo in small animals with high spatial and temporal resolution that other intravital imaging modalities cannot achieve. The new core microscope is critically needed by a large research base of 14 NIH-funded investigators from four USC schools/campuses including the Keck School of Medicine, School of Pharmacy, Davis School of Gerontology, and the Herman Ostrow School of Dentistry. The proposed equipment will be used by these core investigators from seven major user groups/themes, including kidney, liver, stem cells, lung & lacrimal gland, brain, cancer, and dental imaging. The new microscope will make a substantial difference in USC's research activities and fulfill an unmet need for deep tissue intravital quantitative imaging for the study of organ functions in vivo in health and disease. The Zilkha Neurogenetic Institute, which is the academic unit housing this core is fully committed to the ongoing maintenance and operation of this new multiphoton microscope. The USC Multi-Photon Microscopy Core is an integral part of the Center of Excellence in Cell & Tissue Imaging, and the new microscope purchase will meet a strategic need expressed in the USC Plan for Science and Technology Facilities by providing instrumentation and collaborative expertise for biomedical and engineering research aimed at improving human health. Therefore, the USC Core Instrumentation Fund is providing significant cost-share ($200,000) toward the acquisition of this new microscope. Research supported by this core and new instrumentation will lead to our better understanding of the miracle of the brain, the normal functioning of the body's organs, the discovery of disease mechanisms and biomarkers, testing novel pharmaceuticals and biomedical devices, and the application of stem cells and regenerative medicine to cure degenerative disorders, and will help to keep USC at the leading edge of biomedical research.

USC 共享资源的多光子显微镜替代品 提交此提案是为了购买新型高端 Leica SP8 AOBS 多光子相机 用于 Keck 多光子显微镜核心的 1300 nm 红外激光显微镜 南加州大学医学院。新的显微镜将取代旧的、不能使用的显微镜 无法修复且制造商不再提供服务的型号。新的 显微镜将是一个独特的系统，致力于提供超灵敏的深层组织 小动物体内完整活体器官的高时空成像 其他活体成像方式无法达到的分辨率。新核心 由 14 名 NIH 资助的研究人员组成的大型研究基地迫切需要显微镜 来自四所南加州大学学校/校区，包括凯克医学院、 药房、戴维斯老年学学院和赫尔曼奥斯特罗牙科学院。 拟议的设备将由来自七个主要研究机构的核心研究人员使用 用户组/主题，包括肾脏、肝脏、干细胞、肺和泪腺、大脑、 癌症和牙科成像。新的显微镜将带来巨大的改变 南加州大学的研究活动并满足深部组织活体定量方面未满足的需求 成像用于研究健康和疾病中的体内器官功能。齐尔卡 神经遗传学研究所是该核心的学术单位，完全致力于 这种新型多光子显微镜的持续维护和操作。南加州大学 多光子显微镜核心是细胞与成像卓越中心的组成部分 组织成像和新购买的显微镜将满足战略需求 南加州大学科学技术设施计划中通过提供 生物医学和工程研究的仪器和协作专业知识 旨在改善人类健康。因此，USC 核心仪器基金是 为收购这一新设备提供大量成本分摊（200,000 美元） 显微镜。由该核心和新仪器支持的研究将导致我们 更好地了解大脑的奇迹、身体的正常功能 器官、疾病机制和生物标志物的发现、测试新的 药品和生物医学器械，以及干细胞和生物医学的应用 再生医学治疗退行性疾病，并将有助于南加州大学保持领先地位 生物医学研究的前沿。

项目成果

New Endothelial Mechanisms in Glomerular (Patho)biology and Proteinuria Development Captured by Intravital Multiphoton Imaging.

活体多光子成像捕获的肾小球（病理）生物学和蛋白尿发展中的新内皮机制。

• 发表时间: 2021
• 作者: Gyarmati, Georgina;Jacob, Chaim O;Peti
• 通讯作者: Peti

The macula densa prorenin receptor is essential in renin release and blood pressure control.

致密斑肾素原受体对于肾素释放和血压控制至关重要。

• DOI: 10.1152/ajprenal.00029.2018
• 发表时间: 2018-09-01
• 期刊: American journal of physiology. Renal physiology
• 作者: Anne Riquier;A. Sipos;Á. Prókai;S. Vargas;L. Toma;Elliott J. Meer;Karie G. Villanueva;Jennifer C M Chen;Georgina Gyarmati;Christopher Yih;E. Tang;Bahram Nadim;Sujith Pendekanti;I. Garrelds;G. Nguyen;A. Danser;J. Peti
• 通讯作者: J. Peti

Aldosterone induces albuminuria via matrix metalloproteinase-dependent damage of the endothelial glycocalyx.

醛固酮通过基质金属蛋白酶依赖性内皮糖萼损伤诱导白蛋白尿。

• 发表时间: 2019
• 影响因子: 19.6
• 作者: Butler, Matthew J;Ramnath, Raina;Kadoya, Hiroyuki;Desposito, Dorinne;Riquier;Ferguson, Joanne K;Onions, Karen L;Ogier, Anna S;ElHegni, Hesham;Coward, Richard J;Welsh, Gavin I;Foster, Rebecca R;Peti;Satchell, Simon
• 通讯作者: Satchell, Simon

Long-Term Cell Fate Tracking of Individual Renal Cells Using Serial Intravital Microscopy.

使用连续活体显微镜对单个肾细胞进行长期细胞命运追踪。

• 发表时间: 2020
• 作者: Schiessl, Ina Maria;Fremter, Katharina;Burford, James L;Castrop, Hayo;Peti
• 通讯作者: Peti

A new view of macula densa cell microanatomy.

致密黄斑细胞显微解剖学的新观点。

• 发表时间: 2021
• 作者: Gyarmati, Georgina;Shroff, Urvi Nikhil;Riquier;Kriz, Wilhelm;Kaissling, Brigitte;Neal, Christopher R;Arkill, Kenton P;Ahmadi, Nariman;Gill, Inderbir S;Moon, Ju;Desposito, Dorinne;Peti
• 通讯作者: Peti