Anti-tumor mechanism of a dietary and herbal mixture

饮食和草药混合物的抗肿瘤机制

• 批准号: 6818852
• 负责人: LU-HAI WANG
• 金额: $ 25.43万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6818852
• 关键词: alternative medicine; angiogenesis; apoptosis; biological signal transduction; biotherapeutic agent; cell growth regulation; dietary supplements; disease /disorder model; enzyme activity; histopathology; laboratory mouse; male; metastasis; methanol; neoplasm /cancer invasiveness; neoplasm /cancer nutrition therapy; neoplastic cell; neoplastic growth; nonhuman therapy evaluation; nutrition aspect of cancer; nutrition related tag; plant extracts; protein tyrosine kinase; small cell lung cancer; vegetables

DESCRIPTION (provided by applicant) Recently a "Selected Vegetable and Herbal Mix (SV)" was shown to prolong the median survival time (MST) of advanced non-small cell lung cancer (NSCLC) patients 3-fold compared to controls in two small scale phase 1/11clinical trials. Initial laboratory animal study using the L1 lung adenocarcinoma model in BALB/c mice showed that combination of two of the active ingredients of SV was able to inhibit up to 80% of the tumor growth. The SV consists of a mixture of whole plants extracts solely from natural, commonly available vegetables and plants that have long been used in herbal medicine. The ingredients of SV were selected for their known anti-viral, anti-tumor and immune enhancing activities. The hypothesis is that the combined biochemical and biological effects of those active molecules are able to endow one with enhanced immune and tumor suppressive activities. The objectives of this proposal are to compare the anti-tumor efficacy of SV and its water or methanol extracts (SVE) and to elucidate the cellular and molecular mechanism for their anti-tumor effect. The specific aims are: 1) to examine the anti-tumor efficacy of SV and its extracts; 2) to investigate the effect of SV and its extracts on tumor development at cellular level and in various histopathological properties and 3) to examine the effect of SV and its extracts on molecular signaling pathways regulating growth and apoptosis of normal and tumor cells. The L1-BALB/c model will be used to explore the effect of SV and its extracts on tumor cell growth, apoptosis, metastasis and angiogenesis, as well as to identify molecular basis for the anti-tumor effect of SV. Various signaling pathways and molecules regulating the growth and apoptosis of normal and tumor cells will be examined in control and SV treated mice. SV is a safe, non-toxic dietary mixture that can be taken concurrently with other cancer therapeutic regimens. This study shall provide information for optimizing the preparation and formulation of SV and to identify its most significant anti-tumor activities that could reveal potential targets for further development or improvement of anti-tumor agents. The results could also lead to the development of in vitro assays for quality control of the SV preparation. As an anti-cancer food supplement, although the efficacy of SV has so far been shown mainly in the NSCLC patients, based upon its designed anti-tumor and immune enhancing activities, it is anticipated that SV should have a wide spectrum of anti-tumor efficacy

描述（由申请人提供） 最近，与两项小规模1/11临床试验中的对照组相比，最近显示出一种“选定的蔬菜和草药混合物（SV）”，可延长晚期非小细胞肺癌（NSCLC）患者的中位生存时间（MST）3倍。在BALB/C小鼠中使用L1肺腺癌模型的最初实验室动物研究表明，SV的两种活性成分的组合能够抑制多达80％的肿瘤生长。 SV由整个植物提取物的混合物组成，仅来自长期用于草药的天然，常用的蔬菜和植物。 SV的成分被选择用于其已知的抗病毒，抗肿瘤和免疫增强活性。假设是，这些活性分子的生化和生物学效应共同能够赋予免疫和肿瘤抑制活性增强的生化和生物学作用。该提案的目标是比较SV及其水或甲醇提取物（SVE）的抗肿瘤功效，并阐明细胞和分子机制的抗肿瘤作用。具体目的是：1）检查SV及其提取物的抗肿瘤功效； 2）研究SV及其提取物对细胞水平和各种组织病理学特性的肿瘤发育的影响； 3）检查SV及其提取物对调节正常和肿瘤细胞生长和凋亡的分子信号通路的影响。 L1-BALB/C模型将用于探索SV及其提取物对肿瘤细胞生长，凋亡，转移和血管生成的作用，并确定SV抗肿瘤作用的分子基础。调节正常细胞和肿瘤细胞生长和凋亡的各种信号通路和分子将在对照和SV处理的小鼠中检查。 SV是一种安全的，无毒的饮食混合物，可以与其他癌症治疗方案同时服用。这项研究应提供用于优化SV制备和制定的信息，并确定其最重要的抗肿瘤活性，这些活动可能揭示了进一步开发或改善抗肿瘤药物的潜在目标。结果还可能导致开发体外测定法，以控制SV制备的质量控制。作为一种抗癌食品补充剂，尽管迄今为止SV的功效主要在NSCLC患者中显示在其设计的抗肿瘤和免疫增强活动中，但预计SV应该具有广泛的抗肿瘤功效。