LC Microdispenser for High Resolution MALDI-TOF Analysis

用于高分辨率 MALDI-TOF 分析的 LC 微量分配器

• 批准号: 6951484
• 负责人: Patrick W Cooley
• 金额: $ 32.77万
• 依托单位: MICROFAB TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2006-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6951484
• 关键词: biomedical automation; biomedical equipment development; fungal proteins; high throughput technology; liquid chromatography; liquid chromatography mass spectrometry; mass spectrometry; matrix assisted laser desorption ionization; miniature biomedical equipment; proteomics

DESCRIPTION (provided by applicant): We will construct a LC Microdispenser system for rapid liquid chromatography and high throughput loading of digested protein samples onto MALDI-TOF MS targets. This system will incorporate automated preparation of the reversed phase column, including automatic sample loading and integration into the microdispensing device. The capacity of the multi-fluid print head will be increased to 20 different fluids to increase throughput for multiple samples and/or to allow for different chromatography treatments. This system will provide for the maximum conservation of sample volume combined with the rapid LC separation of peptides, resulting in improved MALDI-TOF MS analysis through a reduction in ion suppression. The function and sensitivity of the LC Microdispensor system will be verified by the detection and peptide mass fingerprinting of low abundance proteins in Saccharomyces cerevisiae. Commercialization efforts will be conducted in parallel with the research efforts, in collaboration with our commercialization partners: Proteome Systems, Ltd., Shimadzu Biotech, and Millipore Corporation. Commercial Applications: High throughput instrumentation that improves the sensitivity and dynamic range during MALDI-TOF MS and results in the peptide mass fingerprinting of low abundance proteins is highly desired in the rapidly developing field of proteomics. Details regarding protein expression and post-translational modifications can reveal processes within a biological system useful for drug discovery and the diagnosis and treatment of disease and cancer.

描述（由申请人提供）：我们将构建一个LC微分散器系统，用于快速液相色谱和将消化蛋白样品的高吞吐量负载到MALDI-TOF MS靶标上。该系统将结合反向相位列的自动制备，包括自动样品加载和集成到微分设备中。多流体打印头的容量将增加到20种不同的流体，以增加多个样品的吞吐量和/或允许不同的色谱处理。该系统将提供最大的样品体积保护，并结合肽的快速LC分离，从而通过减少离子抑制，从而改善了MALDI-TOF MS分析。 LC微分散系统的功能和灵敏度将通过对酿酒酵母中低丰度蛋白的检测和肽质量指纹识别来验证。商业化工作将与研究工作同时与我们的商业化合作伙伴合作：Proteome Systems，Ltd。，Shimadzu Biotech和Millipore Corporation。 商业应用： 高吞吐量仪器在MALDI-TOF MS期间提高灵敏度和动态范围，并导致低丰度蛋白的肽质量指纹识别在蛋白质组学快速发展的领域。有关蛋白质表达和翻译后修饰的详细信息可以揭示有助于药物发现以及疾病和癌症的诊断和治疗的生物系统中的过程。