Protein Quality Early in Life: Mechanisms of Growth and Later Obesity Development

生命早期的蛋白质质量：生长和后期肥胖发展的机制

基本信息

批准号：
9352844
负责人：
Minghua Tang
金额：
$ 13.4万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-14 至 2020-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9352844
关键词：
ATP phosphohydrolase Achievement Affect Age-Months Amino Acids Arginine Bacteroidetes Biological Markers Biometry Birth Body Composition Body fat Branched-Chain Amino Acids Child Nutrition Clinical Skills Colorado Complementary Feeding Data Data Analyses Development Diet Dietary Proteins Domestic Fowls Early Intervention Environment Epidemiology Exposure to Faculty Fatty acid glycerol esters Fermentation Fishes Food Future Genes Glycoside Hydrolases Goals Growth Harvest Homeostasis Impairment Individual Infant Institution Insulin Resistance Insulin-Like Growth Factor I Investigation Iron Knowledge Leadership Life Literature Long-Term Effects Marketing Meat Mediator of activation protein Medical Mentors Metagenomics Microbe Micronutrients Mission Monitor Obesity Outcome Overweight Participant Pattern Play Positioning Attribute Postdoctoral Fellow Prevention Proteins Protocols documentation Public Health Randomized Controlled Trials Recommendation Regulation Research Research Methodology Research Training Ribosomal RNA Risk Risk Factors Role Sampling Serum Solid Source Statistical Methods Taxonomy Techniques Time Toddler Training Training Programs United States National Institutes of Health Universities Weight Weight Gain Zinc acylcarnitine base career career development cohort doubly-labeled water effective intervention evidence base experience feeding gut microbiome heart disease risk human disease insulin sensitivity meetings metabolomics metagenomic sequencing microbiome modifiable risk multidisciplinary nutrition obesity in children obesity prevention obesogenic prevent programs protein intake randomized trial research and development skills sound total energy expenditure trimethyloxamine

项目摘要

ABSTRACT Rapid weight gain during the first two years of life predisposes to later-in-life obesity. However, there is a fundamental gap in knowledge of what dietary recommendations should be provided to infants and toddlers to promote optimal growth and prevent rapid weight gain. Dietary protein intake is considered a risk factor because it accelerates weight and fat gain in infants and toddlers, based primarily on research of dairy protein. However, the underlying mechanisms are not clear. These findings have been the basis of recent recommendations to reduce protein intake from birth to 24 months. What is not known is whether all protein sources have the same weight-accelerating effect. Our research group conducted a randomized controlled trial in infants consuming a meat- or dairy-based complementary diet from 6 to 12 months of age. Growth data showed that a dairy-based complementary diet, not meat, leads to impaired linear growth and increased risk for being overweight. The objective of this proposed project is to investigate the underlying mechanisms and determine which mediators are predictive for the different growth patterns in this cohort via fecal microbiome/metagenomics, serum metabolomics and doubly labeled water technique. Although the candidate has expertise in pediatric nutrition and conducting randomized controlled trials, training in mechanistic investigations of obesity development and research methodologies are critical to achieve the long-term goal of becoming a leader in pediatric nutrition focusing on implementing effective interventions early in life for optimal growth and obesity prevention. This career development application proposes a 4-year multidisciplinary training program to the candidate, who is a 4th year postdoctoral fellow transitioning to a junior faculty position. A well-developed training plan by the candidate and the mentoring team is in place, including 1) Hands-on experience of microbiome/metagenomics, metabolomics analysis and data interpretation; 2) Hands-on experience of conducting the doubly labeled water protocol for body composition and total energy expenditure in infants and toddlers, plus sample analysis and data interpretation; 3) Regular meetings with mentors to monitor project progress, achievements towards the short- and long-term goals, and didactic trainings; 4) Advanced coursework in microbiome, metabolomics and biostatistics; 5) Training in leadership and program management. University of Colorado Anschutz Medical Campus is world-leading research institution and provides the candidate an excellent research and training environment which facilitates a successful transition to an independent career in pediatric nutrition and early-in-life obesity prevention.

抽象的生命头两年体重快速增加容易导致晚年肥胖。然而，有一个在应向婴幼儿提供哪些饮食建议方面存在根本性的认识差距促进最佳生长并防止体重快速增加。膳食蛋白质摄入量被认为是一个危险因素因为它会加速婴儿和幼儿的体重和脂肪增加，主要基于乳蛋白的研究。然而，其根本机制尚不清楚。这些发现是最近的基础建议从出生到24个月减少蛋白质摄入量。尚不清楚的是是否所有蛋白质来源具有相同的体重加速效果。我们的研究小组进行了一项随机对照研究对 6 至 12 个月大食用肉类或奶制品为主的补充饮食的婴儿进行试验。生长数据显示，以乳制品为主的补充饮食（而不是肉类）会导致线性生长受损并增加超重的风险。该拟议项目的目标是研究潜在机制和通过粪便确定哪些介质可预测该队列中的不同生长模式微生物组/宏基因组学、血清代谢组学和双标记水技术。虽然候选人拥有儿科营养和进行随机对照试验方面的专业知识，接受过以下方面的培训：肥胖发展的机制研究和研究方法对于实现这一目标至关重要成为儿科营养领域领导者的长期目标，重点是实施有效的干预措施在生命早期实现最佳生长和预防肥胖。该职业发展申请向候选人提出了为期 4 年的多学科培训计划，他是一名第四年博士后研究员，正在过渡到初级教职职位。完善的培训计划候选人和指导团队已就位，包括 1) 的实践经验微生物组/宏基因组学、代谢组学分析和数据解释； 2）实际操作经验对婴儿的身体成分和总能量消耗进行双标记水方案和幼儿，以及样本分析和数据解释； 3）定期与导师会面进行监督项目进展、短期和长期目标的成就以及教学培训； 4) 高级微生物组、代谢组学和生物统计学课程； 5) 领导力和计划培训管理。科罗拉多大学安舒茨医学校区是世界领先的研究机构为候选人提供良好的研究和培训环境，促进成功过渡到儿科营养和早期肥胖预防的独立职业。