REGULATION AND FUNCTION OF FIS IN E COLI

FIS 在大肠杆菌中的调节和功能

• 批准号: 6636115
• 负责人: ROBERT OSUNA
• 金额: $ 24.93万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT ALBANY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6636115
• 关键词: DNA binding protein; Escherichia coli; bacterial proteins; gel electrophoresis; gel mobility shift assay; gene deletion mutation; genetic regulation; molecular cloning; nucleic acid sequence; operon; point mutation; protein structure function; ribosomes; site directed mutagenesis; southern blotting; transfer RNA; transposon /insertion element

DESCRIPTION (adapted from the investigator's abstract): Fis is a small DNA binding protein that plays an important role in a number of cellular processes in E. coli and other enterics, including site-specific recombination systems (e.g. Hin and Gin) and transcription initiation (e.g. rRNA and some tRNA promoters). Its regulation pattern is interesting, with synthesis dramatically increasing from undetectable to 25,000-50,000 molecules/cell following upshift. The goals of this grant are (1) to understand the mechanism of regulation of fis transcription, focusing primarily on the role of initiating nucleotide concentrations (CTP), IHF, and Fis itself on the activity of the fis promoter; (2) to determine what other protein products are controlled by Fis; and (3) to understand the determinants of DNA recognition by Fis.

描述（改编自调查员的摘要）：FIS是一个小的DNA 在许多细胞过程中起重要作用的结合蛋白 在大肠杆菌和其他Enterics中，包括特定地点的重组系统 （例如Hin和Gin）和转录开始（例如RRNA和某些tRNA 发起人）。它的调节模式很有趣，合成很大 在上升后，从无法检测到25,000-50,000分子/细胞。 这笔赠款的目标是（1）了解监管的机制 FIS转录，主要集中于启动核苷酸的作用 浓度（CTP），IHF和FIS本身对FIS启动子的活性； （2）确定其他蛋白质产品由FI控制； （3）到 了解FIS识别DNA的决定因素。