RNA Isolation from Heart Samples for Expression Analysis

从心脏样本中分离 RNA 用于表达分析

• 批准号: 6582790
• 负责人: KAREN LYNN SONDERGAARD
• 金额: $ 34.05万
• 依托单位: GWATHMEY, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6582790
• 关键词: RNA; age difference; biotechnology; chemical information system; data collection methodology /evaluation; data management; gender difference; gene expression; genetic techniques; heart failure; human tissue; information systems; laboratory rat; medical records; myocardium disorder; nucleic acid purification; nucleic acid quantitation /detection; patient /disease registry; polyadenylate

DESCRIPTION (provided by applicant): The overall objective of this study is to determine the changes in gene expression responsible for the development of endstage heart failure. The first step will be to construct two interactive databases 1) for human heart, vascular and skeletal muscle samples and 2) for similar samples from rodent models of heart failure. Gwathmey, Inc has collected hearts and blood vessels, and all the corresponding medical records from human samples that are stored as paper copies only. The investigators propose to develop a database to aid in the management of the tremendous amount of medical information that is available with each human heart. The proposed approach will make it possible for us to access all patients' records according to diagnosis, age, gender, existing medical conditions, medications, race, serology, and other specific clinical tests. Also the investigators will create a database of commonly used rat models of heart failure for comparison. Similarly they will be able to recall heart samples from animal models based on age, gender, and severity of heart failure as well as cause of the heart disease. The human database will allow for continued expansion with an on-line update upon receiving new heart tissue that occurs on a weekly basis. The second goal of this study is to extract total and polyA RNA from all of the normal and diseased heart samples. Initially, the investigators intend to extract RNA from about 400 left ventricular control and myopathic human and rodent hearts that will represent the most common disease states (dilated, ischemic and hypertensive myopathies), as well as appropriate controls. The investigators will examine the quality, purity and quantity of RNA obtained and enter it into both databases so that they can keep a running log of the results. The human and rodent heart databases, in combination with the extensive collection of high quality RNA, will be an extremely valuable research tool and product available to the investigators and clients for innovative studies of differential gene expression during heart failure.

描述（由申请人提供）：这项研究的总体目的是确定导致终阶段心力衰竭发展的基因表达的变化。第一步是构造两个交互式数据库1）人心脏，血管和骨骼肌样本以及2）来自心力衰竭啮齿动物模型的类似样品。 Gwathmey，Inc已收集了心脏和血管，以及来自人类样品的所有相应医疗记录，这些病历仅作为纸张副本存储。研究人员建议开发一个数据库，以帮助管理每个人心脏可用的大量医疗信息。提出的方法将使我们有可能根据诊断，年龄，性别，现有医疗状况，药物，种族，血清学和其他特定临床测试来访问所有患者记录。研究人员还将创建一个常用的心力衰竭大鼠模型的数据库，以进行比较。同样，他们将能够根据年龄，性别和心力衰竭以及心脏病的原因回忆起动物模型中的心脏样本。人类数据库将允许继续扩展，并在每周收到新的心脏组织时在线更新。这项研究的第二个目标是从所有正常和患病的心脏样本中提取总和Polya RNA。最初，研究人员打算从约400个左心室控制和肌病性人和啮齿动物心脏中提取RNA，这些人和啮齿动物心脏将代表最常见的疾病状态（扩张，缺血和高血压肌病），以及适当的控制。研究人员将检查获得的RNA的质量，纯度和数量，并将其输入两个数据库，以便他们可以保持结果的运行日志。人类和啮齿动物心脏数据库结合了广泛的高质量RNA的收集，将成为研究人员和客户可用于心力衰竭期间差异基因表达的创新研究的极其有价值的研究工具和产品。