Identifying Pathogenic Viruses on Microfluidic Platform

在微流控平台上识别病原病毒

• 批准号: 6689126
• 负责人: LOUIS H STRONG
• 金额: $ 10万
• 依托单位: RADIATION MONITORING DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2005-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6689126
• 关键词: G protein; biosensor device; method development; technology /technique development; virus; virus load

Abstract. Species-level Identification of Pathogenic Viruses on a Microfluidics Platform Radiation Monitoring Devices, Inc. (RMD) proposes to develop a field-portable, low power and inexpensive instrument for the identification of pathogenic viruses which are collected by an aerosol concentrator. It will incorporate modular, microfluidic platforms to capture and concentrate the sample for a rapid, two-stage analysis. The first employs pathogen capture on antibody decorated, paramagnetic nanospheres and will provide rapid, preliminary warning based on the hydrodynamic properties of the nanoparticles when the targeted viruses are bound. This stage will monitor the relaxation of their magneto-optical birefringence and will trigger an alarm when the birefringence relaxation rate drops off dramatically. The methodology allows for continuous surveillance over virtually unlimited periods. The second stage will provide either confirmation or correction to the preliminary identification. It is based on the ability to interrogate the captured viral genome with molecular beacon probes which become fluorescent when they hybridize with specific nucleotide sequences. The biosensor will integrate the two stages of sample transfer, preparation and analysis in a microchannel architecture. Employing both proportional mode Avalanche Photodiode (APD) detectors and Geiger-mode pAPDs in array format, high throughput with parallel processing of multiple probes is possible. Sensitivity to low virus titers is afforded by the single photon sensitivity of these detectors. The proposed viral typing instrument will fill the void in field portable instruments that are available for both military and civilian assessment of pathogen risk.

抽象的。在微流体平台上对病原病毒的物种水平鉴定 辐射监测设备（RMD）提议开发一种可识别的，低功率和廉价的仪器，以鉴定由气溶胶浓缩剂收集的致病病毒。它将结合模块化的微流体平台，以捕获和集中样品进行快速的两阶段分析。第一个使用病原体捕获在装饰，顺磁性纳米球的抗体上，并将根据靶向病毒结合时根据纳米颗粒的流体动力特性提供快速的初步警告。此阶段将监视其磁光双折射的放松，并在双重放松率急剧下降时触发警报。该方法允许在几乎无限的时期内进行连续监视。第二阶段将为初步识别提供确认或校正。它基于用分子信标探针询问被捕获的病毒基因组的能力，这些探针与特定的核苷酸序列杂交时会变成荧光。生物传感器将在微通道体系结构中整合样品转移，制备和分析的两个阶段。可以采用比例模式的雪崩光电二极管（APD）检测器和Geiger-Mode Papds以阵列格式，高吞吐量具有多个探针的平行处理。这些检测器的单个光子灵敏度使对低病毒滴度的敏感性得到了敏感性。拟议的病毒打字工具将填充现场便携式工具中的空隙，这些工具可用于军事和平民对病原体风险的评估。