Plasmon-Resonant Nanorods as Contrast Agents

等离激元共振纳米棒作为造影剂

• 批准号: 6708646
• 负责人: Alexander Wei
• 金额: $ 42.05万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6708646
• 关键词: bioimaging /biomedical imaging; biomarker; biomedical equipment development; contrast media; fiber optics; hamsters; hyperthermia; nanotechnology; noninvasive diagnosis; optical tomography; phantom model; surface plasmon resonance

DESCRIPTION (provided by applicant): An integrated, minimally invasive approach will be developed toward the detection, diagnosis, and treatment of cells and tissues in diseased states, using emerging biomedical imaging methods (optical coherence tomography, or OCT) and novel contrast agents (functionalized gold nanorods). OCT is capable of cellular-resolution imaging in the near infrared (NIR) and has the potential to diagnose abnormal cells and tissues at an early stage of disease, given sufficient contrast. Anisotropic gold nanorods exhibit tunable plasmon responses and can be engineered for strong absorption or scattering at select NIR frequencies, thereby enhancing the sensitivity and scope of OCT and related technologies. New OCT methods will be developed based on the enhanced optical contrast of anisotropic nanorods in isolated or aggregate forms. Gold nanorods with strong and selective NIR extinction will be functionalized with biomolecular ligands for selective targeting of tissues with abnormal biomarker expression. These will serve as site-directed optical labels by binding to cell-surface markers or by cell uptake. Nanorods with magnetic tips may provide additional positional and orientational control within the tissue, and enhance the development of a novel, dynamic OCT method. Metal nanorods can also support extremely large absorption cross-sections and generate highly localized thermal gradients, and can be used to enhance optically-induced hyperthermia at low radiation power. The proposed nanorod-enhanced OCT imaging system will first be applied toward tissue phantoms, then toward the in vivo detection and treatment of tumor cells by local hyperthermia using a hamster cheek pouch model. The OCT-nanorod technology is anticipated to have broad application as an integrated, noninvasive system for medical concerns which require image-guided intervention.

描述（由申请人提供）：使用新兴的生物医学成像方法（光学相干断层扫描或OCT）和新颖的对比剂（新型对比剂）（新的生物医学成像方法）和病态状态中细胞和组织的检测，诊断和治疗将开发出一种综合的，微创的方法（诊断和治疗）（功能化的金纳米棒）。 OCT能够在近红外（NIR）中进行细胞分辨率成像，并有可能在疾病的早期诊断异常的细胞和组织，并且给定足够的对比度。各向异性金纳米棒表现出可调的等离子体反应，可以在精选的NIR频率下进行强大的吸收或散射设计，从而增强了OCT和相关技术的灵敏度和范围。 新的OCT方法将根据各向异性或骨料形式的各向异性纳米棒的光学对比度增强。具有强和选择性NIR灭绝的金纳米棒将用生物分子配体功能化，以选择性靶向具有异常生物标志物表达的组织。这些将通过与细胞表面标记或通过细胞摄取结合作为位置定向的光标记。具有磁性尖端的纳米棒可以在组织内提供其他位置和定向控制，并增强新型动态OCT方法的发展。 金属纳米棒还可以支持极大的吸收横截面并产生高度局部的热梯度，可用于在低辐射功率下增强光学诱导的高温。拟议的纳米棒增强OCT成像系统将首先应用于组织幻像，然后使用仓鼠脸颊袋模型，通过局部高温对体内检测和治疗肿瘤细胞。预计Oct Nanorod技术将在需要图像引导的干预措施的医学问题上作为综合的无创系统具有广泛的应用。