Dissection of a Drosophila Polycomb Response Element

果蝇多梳响应元件的解剖

• 批准号: 6631315
• 负责人: Welcome BENDER
• 金额: $ 4.03万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-15 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6631315
• 关键词: Drosophilidae; Europe; arthropod genetics; chromosomes; functional /structural genomics; gene conversion; gene deletion mutation; gene expression; gene induction /repression; gene interaction; genetic regulatory element; genetic screening; immunocytochemistry; phenotype; plasmids; protein localization; protein protein interaction; protein structure function; transposon /insertion element

DESCRIPTION (provided by applicant) Many genes that respond to developmental cues must maintain their responding states long after the cues disappear. In Drosophila, the maintenance of repressed states of many developmentally important genes is accomplished by a protein called POLYCOMB, and by interacting proteins of the Polycomb Group. Homologous proteins are found in mammals, where they appear to have analogous functions. The long-term repression by the Polycomb Group is accompanied by a change in chromosome structure, making it less accessible to transcription factors and polymerases. The Polycomb Group proteins act through DNA sites called Polycomb Response Elements. The best examples of such sites are in the Drosophila bithorax complex, a cluster of genes that control segmental differentiation. Although one of these sites has been localized to a segment of about 1500 base pairs, it is not yet clear how it regulates the genes of the bithorax complex, because there are no mutations that surgically target this Polycomb Response Element. Our goal is to use gene conversion to generate a series of small deletions in and around this site, and to study the consequences for the genes of the bithorax complex. We hope to learn if the Polycomb Response Element is required for activation as well as repression, how the element receives its initial cue, and whether it is redundant or cooperative with other such elements in the bithorax complex. The deletions may permit a functional subdivision of the element, and further conversion experiments will target binding sites for particular Polycomb Group proteins. A second Polycomb Response Element in the bithorax complex will also be dissected by gene conversion, and its properties compared. This application is for a Fogarty International Research Collaboration Award. The research will be done primarily in Hungary at the Biological Research Center of the Hungarian Academy of Sciences, in collaboration with L¿szl¿ Sipos, as an extension of NIH grant #R01 GM28630-21.

描述（由申请人提供） 许多对发育意见的反应必须在许多发育中的d状态下保持其反应状态。组伴随着E染色体，使其对转录因子和多聚体的访问较低。 多肉子蛋白通过称为多康姆响应元素的位点目的是使用基因转换来产生一系列位置和周围的小删除，并研究Bithorax复合体的基因。收到其初始提示，无论是冗余还是与Bithorax复合物中的其他元素合作。 该申请是Fogarch国际研究合作奖。 szl¿ SIPOS，作为NIH赠款＃R01 GM28630-21的扩展。