Biogenesis Of The Salmonella-containing Vacuole

含沙门氏菌液泡的生物发生

• 批准号: 6669987
• 负责人: Olivia Steele-Mortimer
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6669987
• 关键词: Salmonella; Salmonella infections; actins; cell biology; cell membrane; confocal scanning microscopy; cytoskeleton; electron microscopy; endoplasmic reticulum; fluorescence microscopy; gastroenteritis; gene expression; histogenesis; membrane transport proteins; molecular cloning; phagocytosis; secretion; stainings; tissue /cell culture; vesicle /vacuole

Salmonella sp are facultative intracellular bacteria that reside and replicate within a vacuole (SCV). The biogenesis of the SCV is determined by the bacteria and involves effector proteins that are translocated into the host cell via Type III secretion systems. Invasion occurs as a result of localized membrane ruffles that are due to local rearrangement of the actin cytoskeleton. This process is induced by the co-operative effectives of several translocated effector proteins. Membrane ruffles contain large amounts of membrane that must be rapidly delivered. It has been assumed, since only endocytic markers are detected in SCVs, that the biosynthetic/secretory pathway is not involved in this process. In collaboration with Michel Desjardins at the University of Montreal, we have recently shown that the SCV rapidly acquires Endoplasmic Reticulum membranes and indeed appears contiguous with the ER. Thus it is possible that the ER is contributing membrane content to ruffles at the plasma membrane. Indeed, in phagocytic cells, ER membrane can be seen to be contiguous with nascent phagosomes containing either latex beads or Leishmania.

沙门氏菌SP是驻留在液泡（SCV）内的辅助细胞内细菌。 SCV的生物发生由细菌确定，涉及通过III型分泌系统转移到宿主细胞中的效应蛋白。由于肌动蛋白细胞骨架的局部重排而导致的局部膜荷叶边发生。该过程是由几种易位效应蛋白的合作有效性引起的。膜荷叶边包含大量必须迅速传递的膜。已经假定，由于仅在SCV中检测到内吞标记，因此生物合成/分泌途径不参与此过程。与蒙特利尔大学的米歇尔·德斯贾丁斯（Michel Desjardins）合作，我们最近表明，SCV迅速获得内质网膜，并且确实与ER相邻。因此，ER可能会为质膜的荷叶边贡献膜含量。实际上，在吞噬细胞中，可以看出ER膜与含有乳胶珠或利什曼原虫的新生吞噬体连续。