MOLECULAR ANALYSIS OF OLFACTORY PRESYNAPIC RECEPTORS

嗅觉突前受体的分子分析

• 批准号: 6622504
• 负责人: Bettye Hollins
• 金额: $ 7.24万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-15 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6622504
• 关键词: GABA receptor; Malacostraca; alternatives to animals in research; antiserum; cell line; chemoreceptors; electrophysiology; gamma aminobutyrate; human genetic material tag; immunocytochemistry; immunoprecipitation; inhibitor /antagonist; molecular cloning; neural information processing; neuropharmacology; neuroregulation; odors; olfactions; olfactory stimulus; protein structure; receptor expression; recombinant proteins; stimulus /response; synapses

DESCRIPTION (provided by applicant): The long-term goal of this application is to further the understanding of the role of inhibitory receptors in information processing. Information about odors is transduced into electrical impulses by olfactory receptor neurons (ORNs) and transmitted to the central nervous system for further processing. Recent studies indicate that in some species, the output of ORNs is modified by the activation of inhibitory receptors expressed in the afferent terminals of the receptor cells. Although the effect of the inhibition on receptor cell signaling is unclear, the activity of these receptors is likely to critically affect the synaptic strength of contacts between ORNs and their targets in the brain. This application seeks to investigate the molecular nature of these receptors in the olfactory system of the American lobster, Homarus americanus. In the lobster, presynaptic inhibition of ORNs is produced by ionotropic receptors that mediate the effects of gamma aminobutyric acid (GABA). One candidate subunit of this receptor is the recently isolated cDNA that encodes an ionotropic GABA receptor subunit (lob GABA 1). The preliminary data show that lob GABA 1 is highly expressed in lobster ORNs. Subunit specific antisera will be prepared and used in immunocytochemical studies to determine the location of expression of lob GABA 1. Additional subunits that comprise this receptor will also be isolated and the physiological and pharmacological properties of the co-assembled subunits will be examined. Understanding how inhibitory receptors function in sensory signaling is important to the overall understanding of how information is processed in the nervous system. The results of this study will establish the molecular identity of the presynaptic receptors inhibiting the output of ORNs in the olfactory system of the lobster and will provide the preliminary data that will be used to investigate the function of the receptors in the processing of odor signals.

描述（申请人提供）：此申请的长期目标是 进一步理解抑制受体在信息中的作用 加工。有关气味的信息通过将其传递到电脉冲中 嗅觉受体神经元（ORNS），并传播到中枢神经系统 用于进一步处理。最近的研究表明，在某些物种中，输出 ORN的抑制受体在 受体细胞的传入末端。虽然抑制作用 在受体细胞信号传导上尚不清楚，这些受体的活性可能是 严重影响Orn及其之间接触的突触强度 大脑中的目标。该应用旨在研究分子性质 这些受体在美国龙虾嗅觉系统中 美洲。在龙虾中，ORN的突触前抑制是由 介导γ氨基丁酸（GABA）作用的离子受体。 该受体的一个候选亚基是最近分离的cDNA 编码一个离子GABA受体亚基（LOB GABA 1）。初步数据 证明Lob Gaba 1在龙虾Orns中高度表达。亚基特异性 抗血清将被准备并用于免疫细胞化学研究，以确定 LOB GABA 1的表达位置。 受体也将被分离出来，生理和药理 将检查共同组装亚基的特性。了解如何 抑制受体在感觉信号中的功能对整体很重要 了解在神经系统中如何处理信息。结果 这项研究将建立突触前受体的分子身份 抑制龙虾嗅觉系统中ORN的输出，将 提供将用于调查功能的初步数据 气味信号处理中的受体。