Optimization of a murine model of the human vaginal microbiome: A much needed tool for understanding the connections between vaginal microbes and host health

人类阴道微生物组小鼠模型的优化：了解阴道微生物与宿主健康之间联系的急需工具

• 批准号: 9168798
• 负责人: Seth T Walk
• 金额: $ 7.2万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-22 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9168798
• 关键词: Affect; Age; Animal Model; Atrophic; Bacteria; Bacterial Vaginosis; Biological Models; Burn injury; Clinical; Collection; Communities; Conditioned Culture Media; Data; Development; Diagnosis; Dimethyl Sulfoxide; Disease; Dose; Ensure; Environment; Estradiol; Exhibits; Exogenous Factors; Funding; Germ-Free; Glycerol; Glycogen; Gnotobiotic; Health; Hemorrhage; Human; Human Subject Research; Incubators; Inflammatory; Inflammatory Response; Lactobacillus; Lead; Literature; Long-Term Effects; Low Birth Weight Infant; Maintenance; Menopause; Microbe; Modeling; Morbidity - disease rate; Mothers; Mouse Strains; Mus; Neonatal; Pain; Pelvis; Play; Postmenopause; Pregnant Women; Preparation; Prevotella; Procedures; Protocols documentation; Research; Research Personnel; Risk; Role; Sampling; Seeds; Sexually Transmitted Diseases; Specimen; Streptococcus; Swab; Symptoms; System; Urinary tract; Vagina; Vaginal Diseases; Virus; Woman; clinically relevant; human subject; innovation; irritation; metabolome; microbial; microbial community; microbiome; microbiota; mouse model; neonatal morbidity; neonate; parity; pathogenic bacteria; prevent; reproductive; tool; vaginal lactobacilli; vaginal microbiome

Executive Summary Vaginal microbes present the primary barrier to the invasion and outgrowth of pathogenic and potentially pathogenic bacteria and viruses. Disruptions of the typically dominant vaginal lactobacilli are associated with numerous biomedically important diseases, and increased risks of sexually transmitted infections (STI). Understanding the mechanism(s) underpinning disruption of vaginal lactobacilli has been limited due to the lack of a physiologically relevant animal model. Recently, we found that we can replicate human vaginal microbial communities within germ free mice. The current application seeks funding to further develop and optimize this model, and produce SOP for the collection and storage of human specimens.

执行摘要 阴道微生物是病原微生物入侵和生长的主要屏障。 潜在致病细菌和病毒。通常占主导地位的阴道的破坏 乳酸菌与许多生物医学上重要的疾病有关，并且增加了以下疾病的风险： 性传播感染（STI）。了解破坏的机制 由于缺乏生理相关的动物模型，阴道乳酸杆菌受到限制。 最近，我们发现我们可以在无菌条件下复制人类阴道微生物群落 老鼠。当前的申请寻求资金来进一步开发和优化该模型，并且 制定用于采集和储存人体标本的 SOP。