BIOEFFECTS OF PRENATAL ULTRASOUND EXPOSURE IN MACAQUES

猕猴产前超声暴露的生物效应

• 批准号: 3742058
• 负责人: ALICE F TARANTAL
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3742058
• 关键词: Macaca; binding proteins; blood cell count; embryo /fetus; hematopoietic stem cells; insulinlike growth factor; neutrophil; ultrasound biological effect

The obstetrical applications of ultrasound have expanded during the last four decades , as sophisticated technologies have evolved. Although considered an essential method for assessing fetal status, there remain many gaps in our understanding of the interaction of this imaging modality with fetal tissues. Prior investigations with the monkey have shown that reduced birth weights and transient neutropenias result from frequent prenatal exposure. To further explore these findings, 26 animals were studied (16 exposed, 10 controls; "triple mode"; atl ultramark 9 with hdi~; isptad~645-714 mw/cm2). Exposures were performed every 5 days from gestational day (gd) 21-36 (5 min), every other day from gd 37-60 (5 min), then weekly from gd 61-153q1 (10 min). Fetal blood samples were collected for complete blood counts (CBCS), progenitor assay, circulating insulin-like growth factors (IGF-I, IGF-II) and binding proteins (IGFBP-3). Animals were delivered by cesarean section at term, and body weights, morphometrics, cbcs, and bone marrow aspirates assessed at delivery and postnatally (3 months). Fetal neutropenias were observed in exposed animals in addition to reductions in circulating progenitors (colony forming unit-granulocyte-macrophage [CFU-GML). Growth of CFU-GM from bone marrow was exuberant at term, whereas circulating levels were diminished compared to prenatal samples. Exposed animals were smaller at birth; marked reductions in fetal IGFBP-3 were noted. These data suggest that (1) frequent prenatal ultrasound exposure can transiently alter the neutrophil lineage which may be due to enhanced margination and/or organ sequestration, and (2) transient, altered growth patterns may be due to perturbations of the IGF axis. Continued studies with the monkey model will help further our understanding of potential interactions of this imaging modality with developing tissues, and help establish the mechanisms by which biologic effects may occur in developing mammalian systems.

超声的产科应用在最后一次扩大 四十年来，随着复杂技术的发展。 虽然 被认为是评估胎儿状况的重要方法，仍然存在 我们对这种成像相互作用的理解的差距很多 胎儿组织的模态。 先前与猴子的调查 表明出生体重减轻和瞬时中性粒细胞减少。 频繁的产前暴露。 为了进一步探索这些发现，26 研究了动物（16个暴露，10个对照；“三重模式”； ATL HDI〜的Ultramark 9; ISPTAD〜645-714 MW/CM2）。 进行曝光 妊娠日（GD）21-36（5分钟），每隔5天，每隔一天 从GD 37-60（5分钟），然后每周从GD 61-153Q1（10分钟）开始。 胎 收集血液样本以进行全血数（CBC），祖细胞 测定，循环胰岛素样生长因子（IGF-I，IGF-II）和 结合蛋白（IGFBP-3）。 动物是通过剖宫产分割的 在学期，体重，形态计量学，CBC和骨髓抽吸 在交货和产后（3个月）时进行评估。 胎儿中性粒细胞减少症 除了减少循环外，在暴露的动物中观察到 祖细胞（菌落形成单位颗粒细胞 - 巨噬细胞[CFU-GML）。 骨髓中CFU-GM的生长在学期是旺盛的，而 与产前样品相比，循环水平降低。 裸露 出生时动物较小；胎儿IGFBP-3的明显减少为 著名的。 这些数据表明（1）经常进行产前超声暴露 可以瞬时改变中性粒细胞谱系，这可能是由于增强的 边缘和/或器官隔离，以及（2）瞬时生长改变 模式可能是由于IGF轴的扰动。 继续研究 使用猴子模型将有助于我们进一步理解潜力 这种成像方式与发展组织的相互作用，并有助于 建立可能在 开发哺乳动物系统。