MECHANISMS OF CENTRAL PATTERN GENERATION IN GANGLIA

神经节中心模式生成机制

• 批准号: 2261128
• 负责人: Allen Israel Selverston
• 金额: $ 13.59万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-24 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2261128
• 关键词: Malacostraca; biological models; brain regulatory center; dyes; electrophysiology; ganglions; interneurons; mechanoreceptors; micromanipulator; neural information processing; neural plasticity; neuropharmacology; neurotransmitters; proprioception /kinesthesia; sensorimotor system; sensory feedback; slow potential; stomach; synapses; voltage /patch clamp

This proposal is aimed at a systems-level analysis of how the gastric mill central pattern generator (CPG) of lobsters functions as a neuronal network and how it integrates sensory feedback and coordinating central inputs in a complex, plastic manner. While not immediately related to health sciences, the gastric CPG nevertheless is an excellent system for studying basic mechanisms underlying the generation and control of rhythmic behaviors. Since 1969 this work has established the lobster stomatogastric ganglion as the most thoroughly understood neural circuit presently available. The research plan has 3 specific aims: 1. To study the role of synaptic timecourse in the temporal operation of the gastric CPG. Electrophysiological and pharmacological methods will be used to study the transmitters and postsynaptic (possibly metabotropic) mechanisms underlying a recently-discovered class of slow synapses. Pharmacological manipulation will be used to dissect the role of synaptic timecourse in temporal processing within this neural circuit. 3. To examine plasticity of sensorimotor processing in the gastric CPG. Here recent studies of identified mechanosensory feedback loops will be extended. Peptidergic and muscarinic modulators are known to produce a functional reorganization of the gastric circuit. Using electrophysiological techniques to record and manipulate activity unidentified neurons, and a dye-sensitized photinactivation method to ablate specific members of the network, this project will analyze the cellular and synaptic basis of changes in sensorimotor processing within the circuit, induced by neuromodulators. 3. To study the actions on the gastic CPG of a pair of identified, command interneurons that serve to coordinate its rhythmic operation with that of an adjacent motor system. Current-and voltage-clamp studies will be combined with pharmacological techniques to extend the understanding of the transmitters and membrane responses involved in the multiple synaptic actions of these interneurons. These cellular and synaptic properties will be related to a systems-level analysis of how the coordinating inputs alter the function of the whole network.

该建议针对胃磨坊的系统级分析 龙虾的中央模式发生器（CPG）充当神经元网络 以及如何整合感觉反馈和协调中央输入 复杂的塑料方式。 虽然与健康无关 科学，胃CPG仍然是研究的绝佳系统 节奏的产生和控制的基本机制 行为。 自1969年以来，这项工作已建立了龙虾大口径 神经节目前是最彻底了解的神经电路 可用的。 研究计划具有3个具体目标： 1。研究突触时间在时间​​操作中的作用 胃CPG。 电生理和药理方法将是 用于研究发射器和突触后（可能是代谢型） 最近发现的慢慢突触类别的机制。 药理操作将用于剖析突触的作用 该神经回路内的时间处理时间。 3。检查胃CpG中感觉运动处理的可塑性。 这里对已识别的机械感觉反馈回路的最新研究将是 扩展。 已知替代肽和毒蕈碱调节剂会产生 胃回路的功能重组。 使用 记录和操纵活动的电生理技术 身份不明的神经元，以及染料敏化的光学活化方法 消融网络的特定成员，该项目将分析 蜂窝和突触的基础的感觉运动处理变化 该电路，由神经调节剂引起。 3。研究一对已识别的命令的行为CpG的动作 用于协调其节奏操作的中间神经元与 相邻的电机系统。 电流和电压钳研究将是 结合药理技术，以扩展对 发射器和膜反应涉及多个突触 这些中间神经元的作用。 这些细胞和突触特性将 与对协调输入如何改变的系统级分析有关 整个网络的功能。