OXIDATION OF PHENOLIC COMPONENTS OF CIGARETTE TAR

卷烟焦油酚类成分的氧化

• 批准号: 2658810
• 负责人: BRIAN M FREED
• 金额: $ 6.09万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1999-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2658810
• 关键词: T lymphocyte; biomarker; catechols; cell proliferation; chelating agents; glutathione; high performance liquid chromatography; human subject; hydrogen peroxide; hydroquinones; immunosuppressive; lymphocyte; oxidative stress; tobacco

DESCRIPTION The applicant has published that T lymphocytes obtained from lungs of smokers demonstrate reduced proliferative responses to agents such as ConA and, when stimulated, fail to produce the usual amounts of IL-2. It is proposed to determine how oxidative stress in the form of H2O2, which has been shown to be generated in tobacco smoke, affects the immunosuppressive properties of hydroquinone and catechol, both of which are present in tobacco smoke. Low levels of H2O2 will be used to determine how it affects the ability of hydroquinone to suppress T cell activation and proliferation. Experiments will be designed to determine to what extent the iron-chelating properties of catechol protects lymphocytes from the inhibitory effects of H2O2. In addition, measurements will be made to determine to what extent conjugates of parabenzoquinone and glutathione correlate with smoke-induced T cell suppression. In addition, quinone-glutathione levels will be determined by HPLC to determine if this is a useful biomarker for suppressed T cell responses.

描述 申请人已经公布了从肺的肺中获得的T淋巴细胞 吸烟者表现出对CONA等药物的增殖反应减少 而且，如果刺激，则无法产生通常的IL-2。这是 提议确定H2O2形式的氧化应激如何 被证明是在烟草烟中产生的，会影响免疫抑制 羟基酮和儿茶酚的特性，它们都存在于 烟草烟。低水平的H2O2将用于确定其影响如何 氢喹酮抑制T细胞激活和增殖的能力。 实验将被设计为确定铁授予的程度 儿茶酚的特性可保护淋巴细胞免受抑制作用 H2O2。此外，将进行测量以确定在多大程度上 羟基苯苯二甲酸强酮和谷胱甘肽的缀合物与烟雾引起的 T细胞抑制。此外，喹酮 - 谷胱甘肽水平将是 由HPLC确定以确定这是否是抑制的有用的生物标志物 T细胞反应。