3-DIMENSIONAL CALIBRATION TARGET--CONFOCAL MICROSCOPY

三维校准目标--共焦显微镜

• 批准号: 2421210
• 负责人: WALTER P SIEGMUND
• 金额: $ 10万
• 依托单位: TAPERVISION (PARTNERSHIP)
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-15 至 1998-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2421210
• 关键词: biomedical equipment development; chemical standardization; confocal scanning microscopy; fiber optics; fluorescent dye /probe; glass; phantom model

Our long term goal is to develop a phantom for use in calibrating and measuring resolution of confocal microscopes using fiber optic technology. Modern microscopy is increasingly quantitative. Size of the observed objects is critical in a number of areas. Resolution is also a necessary parameter in many measurements, and that now includes resolution along the vertical dimension (axial depth into the sample) direction as well as lateral (in the image plane). Calibration of intensity completes the set of quantities needed to make it possible to compare measurements between microscopes or across time. In the reflectance mode this will be done by using different ranges of index differences. Glasses with fluorescent dopants can provide a calibratory for the confocal microscope (CFM) used in a fluorescent mode (now the most common mode in biologial studies). In this mode the calibratory can also be used to measure registration of the images obtained in two wavelength. This proposal is for the development of a calibration standard tool for all these quantities. We will use TaperVision expertise in fiberoptics technology to achieve the necessary dimensions and tolerances for such a measurement tool. The phase I proposal is to evaluate the feasibility of generating such a calibrating target in the right parameters and to show that intensity calibration and fluorescent mode devices can also be constructed using the same basic technology. POTENTIAL COMMERCIAL APPLICATION: The calibrator will be used by developers and users of CFM's to verify performance and to obtain measurements. There are currently 5000 CFM's on the market and their number and applications are expected to rise sharply.

我们的长期目标是开发用于校准和 使用光纤测量共聚焦显微镜分辨率 技术。 现代显微镜越来越定量。 大小 在许多区域中，观察到的对象至关重要。 解决方案是 在许多测量中也是必要的参数，现在 包括沿垂直尺寸的分辨率（轴向深度进入 样品）方向以及侧面（在图像平面中）。 校准 强度完成了使之成为可能的数量集 比较显微镜之间或跨时间之间的测量。 在 反射模式这将通过使用不同的索引范围来完成 差异。 带有荧光掺杂剂的眼镜可以提供校准 用于在荧光模式下使用的共聚焦显微镜（CFM）（现在 生物学研究中最常见的模式）。 在此模式下 校准也可以用于测量图像的注册 以两个波长获得。 该建议是为了发展 所有这些数量的校准标准工具。 我们将使用 tapervision fibibiboptics技术的专业知识，以实现 这种测量工具的必要维度和公差。 第一阶段提议是评估产生这种A的可行性 在正确的参数中校准目标并显示强度 也可以使用校准和荧光模式设备使用 相同的基本技术。 潜在的商业应用： CFM的开发人员和用户将使用校准器来验证 性能并获得测量。 目前有5000 CFM在市场上及其数量和应用预期 急剧上升。