ARC DETECTION FOR AMPLIFICATION BASED GENETIC TYPING

用于基于扩增的基因分型的电弧检测

基本信息

批准号：
2030097
负责人：
TRUETT C BOLES
金额：
$ 6.68万
依托单位：
MOSAIC TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-15 至 1998-06-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2030097
关键词：
DNA primers biomedical automation biotechnology genetic polymorphism histocompatibility antigens histocompatibility typing human genetic material tag nucleic acid sequence point mutation polymerase chain reaction serotyping

项目摘要

The overall objective of this proposal is to provide a fast and reliable automated method for amplification-based genetic typing of point mutations and allelic single-base changes. The proposed method called ARCTM detection, is designed for analysis of highly polymorphic DNA targets that can be amplified by the polymerase chain reaction (PCR). The method can simultaneously type hundreds of nucleotide positions using the amplified products from a single PCR reaction. The ARCTM process relies on polymerase extension of allele-specific primer pairs that have been immobilized on a solid support. ARCTM detection will provide faster and more reliable typing results than allele-specific hybridization, with less technician labor, and should have broad applications in HLA typing, diagnosis of genetic disease, infectious disease diagnostics, forensics, genetic mapping and mutation analysis. In phase I, the feasibility of the proposed method will be tested by constructing a prototype, twelve-assay, multiplex ARC detector for typing all HLA-DQA1 alleles using a single amplification reaction. PROPOSED COMMERCIAL APPLICATIONS ARC TM detection will have broad applications in HLA typing, blood and plasma screening, genetic disease diagnosis, infectious disease diagnostics, cancer diagnostics, and DNA-based forensics. Our initial products will be HLA typing systems for the tissue-typing and forensic testing industries.

该提案的总体目标是提供快速且可靠的基于扩增的基因分型的自动化方法点突变和等位基因单碱基变化。所提出的方法称为 ARCTM 检测，专为分析高度可通过聚合酶扩增的多态性 DNA 靶标链式反应（PCR）。该方法可以同时输入数百个使用单个扩增产物的核苷酸位置 PCR反应。 ARCTM 过程依赖于聚合酶延伸固定在固体上的等位基因特异性引物对支持。 ARCTM检测将提供更快、更可靠与等位基因特异性杂交相比，分型结果更少技术人员的劳动，并且应该在 HLA 分型中具有广泛的应用，遗传病诊断、传染病诊断、法医学、遗传图谱和突变分析。在第一阶段，将通过构建来测试所提出方法的可行性原型、十二检测、多重 ARC 检测器，用于分型所有使用单一扩增反应的 HLA-DQA1 等位基因。拟议的商业应用 ARC TM 检测将具有广泛的应用前景在 HLA 分型、血液和血浆筛查、遗传疾病诊断、传染病诊断、癌症诊断和基于 DNA 的取证。我们最初的产品将是用于组织分型和法医检测的 HLA 分型系统行业。