Upgrade for a 600 MHz Structural Biology NMR

升级为 600 MHz 结构生物学 NMR

• 批准号: 9075568
• 负责人: JAMES H. PRESTEGARD
• 金额: $ 59.99万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075568
• 关键词: 17 year old; Adopted; Area; Carbon; Communities; Complex; Detection; Development; Faculty; Future; Grant; Heat shock proteins; Immune response; Metabolism; Minor; NMR Spectroscopy; Natural Products; Neoplasm Metastasis; Performance; Polysaccharides; Post-Translational Protein Processing; Proteins; Protocols documentation; Protons; Regulation; Research; Role; Scientist; Signal Transduction; Site; Structure; System; United States National Institutes of Health; Universities; Virulence Factors; Work; base; digital; drug efficacy; improved; instrument; instrumentation; pathogenic bacteria; programs; protein complex; research study; response; stress protein; structural biology

 DESCRIPTION (provided by applicant): An upgrade of an existing 600 MHz NMR spectrometer used primarily for structural biology applications is proposed. The upgrade will replace a 17 year old Varian INOVA console with a new Bruker Avance III HD 600 High Performance Digital NMR Console and an existing cryobay with a Bruker cryoplatform. The upgrade will allow the use of a high sensitivity cryoprobe that is optimized for 13C (and 15N) detection along with 1H and 2H decoupling. The instrument is being placed in a well-staffed and supported NMR facility that serves the University of Georgia (UGA) scientific community, as well as a number of additional scientists throughout the region. The ability to observe 13C directly with high sensitivity will add to the facility a capability that is unique, and one that is becomin increasingly important in the characterization of biomolecular systems. These systems include large proteins or proteins that are parts of large complexes, proteins with intrinsically disordere regions, and natural products (including some glycans) that have significant numbers of non- protonated carbon sites. In addition to this unique capability, the system will execute, with sensitivity comparable or greater than non-cryo systems, the traditional proton detected triple resonance experiments that have become so important to structure determination of biomedically important proteins. In the latter case the more reliable and up-to-date system will add important capacity to serve the growing number of NMR users who pursue NMR-based structural work at UGA. Seven major users and three minor users have provided research descriptions as a part of this proposal. Their projects impact on areas such as: the role of glycans in development and metastasis, protein modifications in response to environmental stress, protein interactions in cell signaling and immune response, regulation of virulence factors in pathogenic bacteria, and improving drug efficacy through regulation of transport and metabolism. Seven of the ten users are currently supported by NIH grants, and we expect additional NIH support as the newly added faculty establish their UGA research programs. The requested instrumentation will allow them that access to NMR instrumentation needed for their current research, and it will allow them to adopt new experimental protocols that allow them to attack ever more complex problems in the future.

 描述（由申请人提供）：建议对主要用于结构生物学应用的现有 600 MHz NMR 波谱仪进行升级。此次升级将用新的 Bruker Avance III HD 600 高性能数字 NMR 控制台和新的 Bruker Avance III HD 600 高性能数字 NMR 控制台取代已有 17 年历史的 Varian INOVA 控制台。带有 Bruker 冷冻平台的现有冷冻室 升级后将允许使用针对 13C（和 15N）检测进行优化的高灵敏度冷冻探针。该仪器与 1H 和 2H 解耦一起被放置在一个人员配备齐全且支持的 NMR 设施中，该设施为佐治亚大学 (UGA) 科学界以及整个地区的许多其他科学家提供了观察 13C 的能力。直接以高灵敏度进行分析将为该设施增添一种独特的能力，这种能力在生物分子系统的表征中变得越来越重要，这些系统包括大蛋白质或作为大复合物一部分的蛋白质，本质上具有蛋白质。无序区域和具有大量非质子化碳位点的天然产物（包括一些聚糖） 除了这种独特的功能之外，该系统还将以与非冷冻系统相当或更高的灵敏度执行传统的质子检测三重。共振实验对于生物医学上重要的蛋白质的结构测定变得如此重要，在后一种情况下，更可靠和最新的系统将增加重要的能力，为佐治亚大学追求基于 NMR 的结构工作的越来越多的 NMR 用户提供服务。七个主要用户和三个次要用户。用户提供了研究描述作为该提案的一部分，他们的项目对以下领域产生影响：聚糖在发育和转移中的作用、响应环境压力的蛋白质修饰、细胞信号传导和免疫反应中的蛋白质相互作用、毒力因子的调节。 致病菌，并通过调节运输和代谢来提高药物疗效，目前 10 个用户中有 7 个得到 NIH 拨款的支持，随着新增加的教员建立他们的 UGA 研究项目，我们预计 NIH 会提供额外的支持。他们当前的研究需要使用核磁共振仪器，这将使他们能够采用新的实验协议，从而使他们能够在未来解决更复杂的问题。

项目成果

Dissolved organic metabolite extraction from high-salt media.

从高盐介质中提取溶解的有机代谢物。

• 发表时间: 2023-04
• 影响因子: 2.9
• 作者: Holderman, Nicole R;Ferrer;Glushka, John;Moran, Mary Ann;Edison, Arthur S
• 通讯作者: Edison, Arthur S

Continuous in vivo Metabolism by NMR.

通过 NMR 进行连续体内代谢。

• 发表时间: 2019
• 作者: Judge, Michael T;Wu, Yue;Tayyari, Fariba;Hattori, Ayuna;Glushka, John;Ito, Takahiro;Arnold, Jonathan;Edison, Arthur S
• 通讯作者: Edison, Arthur S