Non-human primate MHC and KIR Allele Discovery and Typing Technology Development

非人灵长类 MHC 和 KIR 等位基因发现和分型技术开发

• 批准号: 10849594
• 负责人: DAVID O'CONNER, PHD
• 金额: $ 118.61万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2028-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10849594
• 关键词: Alleles; Animals; Biological Assay; Catalogs; Communities; Complementary DNA; Data Analyses; Development; Fc Receptor; Gene Frequency; Genes; Genetic Services; Genomic DNA; Genotype; Goals; Immunoglobulins; Killer Cells; Laboratories; Libraries; MHC Class I Genes; MHC Class II Genes; Macaca fascicularis; Macaca mulatta; National Institute of Allergy and Infectious Disease; Population; Pre-Clinical Model; Receptor Gene; Research; Resolution; Technology; Transplantation; data curation; exome sequencing; gene discovery; genome sequencing; genomic data; genotyping technology; improved; member; nonhuman primate; programs; public database; receptor; technology development; whole genome

The objective of this program is to discover new NHP MHC Class I, MHC Class II, Killer-cell Immunoglobulin like Receptor (KIR), and Fc Receptor (FcR) genes, loci, and alleles; develop genotyping technologies that will remain freely accessible to the NHP research community; provide all sequences generated to the NIAID-designated, publicly available databases. Other important goals are to: i) develop and apply whole genome/whole exome sequencing (WGS, WES respectively) to the MHC, KIR, and FcR regions; and ii) generate and make publicly available supplemental information, including: a) primer and probe definitions used in gene/allele discovery or genotyping; and b) familial relationships of animals used in gene discovery and allele frequency studies. This information will support the development and interpretation of transplantation and other studies in the NHP pre-clinical model

该计划的目标是发现新的 NHP MHC I 类、MHC II 类、杀伤细胞免疫球蛋白样受体 (KIR) 和 Fc 受体 (FcR) 基因、基因座和等位基因；开发 NHP 研究界可免费使用的基因分型技术；将生成的所有序列提供给 NIAID 指定的公开数据库。其他重要目标是： i) 开发并将全基因组/全外显子组测序（分别为 WGS、WES）应用于 MHC、KIR 和 FcR 区域； ii) 生成并公开补充信息，包括： a) 用于基因/等位基因发现或基因分型的引物和探针定义； b) 用于基因发现和等位基因频率研究的动物的家族关系。 该信息将支持 NHP 临床前模型中移植和其他研究的开发和解释