Role of periostin expressing cells in intramembranous bone regeneration

骨膜蛋白表达细胞在膜内骨再生中的作用

基本信息

批准号：
10856528
负责人：
Frank Ko
金额：
$ 6.35万
依托单位：
RUSH UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-01 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10856528
关键词：
Ablation Administrative Supplement Antibodies Area Bone Marrow Ablation Bone Regeneration Bone Tissue Brain Cartilage Cells Collagen Data Dental Care Detergents Distraction Osteogenesis Enzymes Extracellular Matrix Failure Fluorescent Dyes Goals Histologic Image Imaging technology Impairment Implant Implantation procedure Inflammation Knee joint Label Light Limb structure Marrow Microscopy Molecular Morphology Mus Muscle Musculoskeletal Musculoskeletal Diseases Neurons Nociceptors Operative Surgical Procedures Orthopedic Procedures Orthopedics Pain Pain Research Parents Patients Periosteum Permeability Photobleaching Play Process Proteins Refractive Indices Research Rheumatism Role Signal Transduction Skeletal Muscle Solvents Spinal Cord Technology Temporomandibular Joint Tendon structure Therapeutic Time Tissues Visualization angiogenesis antibody conjugate base bone bone fracture repair cohort collagenase imaging approach imaging modality intramembranous bone microscopic imaging novel parent grant periostin preservation skin disorder soft tissue spatiotemporal three-dimensional visualization

项目摘要

Project Summary/Abstract: Intramembranous bone regeneration plays an important role in several orthopaedic and dental procedures such as implant placement, fracture healing, and distraction osteogenesis. Prior studies have examined different processes during intramembranous bone regeneration, such as inflammation, angiogenesis, and bone regeneration. However, an under explored aspect of intramembranous bone regeneration is pain sensing neurons, such as their distribution and morphological changes over the course of intramembranous bone regeneration. A rigorous examination of pain sensing neurons can be achieved by tissue clearing and light sheet microscopy, which allows three-dimensional visualization and quantitation of tissues. However, implementing these approaches have been met with significant technical challenges in musculoskeletal tissues, such as significant autofluorescence and insufficient depth of field due to collagen-rich extracellular matrix. Our preliminary data demonstrate several approaches that overcome these challenges, but further refinement and comparison studies to conventional histological approaches are needed. Therefore, we are proposing this supplement that aims to develop musculoskeletal tissue specific tissue clearing light sheet microscopy that will examine periostin expressing cells and pain sensing neurons during intramembranous bone regeneration. The supplement will address the strategic goal “Goal B: Expand rheumatic, skin, and musculoskeletal disease pain research capacity (cohorts, technologies or data) beyond current research base/collaborators” with a specific scientific area in novel pain imaging technologies for musculoskeletal tissues.

项目摘要/摘要：膜内骨再生在几个骨科和牙科手术，例如植入物的放置，断裂愈合和分心成骨。先前的研究检查了膜内骨再生期间的不同过程，例如炎症，血管生成和骨再生。但是，膜内探索的方面骨骼再生是疼痛感应神经元，例如它们的分布和形态学变化膜内骨再生的过程。严格检查疼痛感应神经元可以是通过组织清除和光片显微镜实现，这允许三维可视化和组织的定量。但是，实施这些方法已得到了重要的技术肌肉骨骼组织的挑战，例如明显的自动荧光和应有的景深不足到富含胶原蛋白的细胞外基质。我们的初步数据展示了几种克服的方法这些挑战，但对常规组织学方法的进一步完善和比较研究是需要。因此，我们提出了旨在发展肌肉骨骼组织特异性的补充剂组织清除光片显微镜将检查表达细胞的周期和疼痛传感神经元在膜内骨再生期间。该补充剂将解决战略目标“目标B：扩展风湿性，皮肤和肌肉骨骼疾病疼痛研究能力（队列，技术或数据）当前的研究基础/合作者”具有新型疼痛成像技术的特定科学领域肌肉骨骼组织。

项目成果

期刊论文数量（2）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Activation of canonical Wnt signaling accelerates intramembranous bone regeneration in male mice.

DOI：
10.1002/jor.25217
发表时间：
2022-08
期刊：
JOURNAL OF ORTHOPAEDIC RESEARCH
影响因子：
2.8
作者：
Ko, Frank C.;Moran, Meghan M.;Ross, Ryan D.;Sumner, D. Rick
通讯作者：
Sumner, D. Rick

Colon epithelial cell-specific Bmal1 deletion impairs bone formation in mice.

结肠上皮细胞特异性 Bmal1 缺失会损害小鼠的骨形成。

DOI：
10.1016/j.bone.2022.116650
发表时间：
2023
期刊：
Bone
影响因子：
4.1
作者：
Ko,FrankC;Jochum,SarahB;Wilson,BrittanyM;Adra,Amal;Patel,Nikhil;Lee,Hoomin;Wilber,Sherry;Shaikh,Maliha;Forsyth,Christopher;Keshavarzian,Ali;Swanson,GarthR;Sumner,DRick
通讯作者：
Sumner,DRick