Center for Photogenomics

光基因组学中心

基本信息

批准号：
8883312
负责人：
JOHN A STAMATOYANNOPOULOS
金额：
$ 200万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-14 至 2016-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8883312
关键词：
Back Biochemical Biological Breeding Cell Nucleus Cells Chemicals Chemistry Chromatin Chromatin Structure Alteration Clinical DNA DNA-Binding Proteins Detection Development Diagnostic Education and Outreach Engineering Enhancers Ensure Environment Foundations Generations Genetic Fingerprintings Genome Genomics Glucocorticoids Human Human Genome Image Image Analysis Imagery Imaging Device Imaging Techniques In Situ Indium Individual Knowledge Laboratories Lateral Marrow Methods Microscope Microscopy Mission Modeling Modification Nuclear Nuclear Localization Signal Nucleic Acid Regulatory Sequences Oligonucleotides Optics Pattern Postdoctoral Fellow Property Quantum Dots RNA Regulatory Element Reporting Reproducibility Research Research Personnel Resolution Site Solid Specimen Speed Structure Techniques Technology Tissues Translations base cell type clinically relevant doctoral student fluorophore in vivo innovation instrument instrumentation nanocrystal new technology non-genomic novel peripheral blood pre-doctoral programs public health relevance tissue/cell preparation transcription factor

项目摘要

DESCRIPTION (provided by applicant): The mission of the Center for Photogenomics is to develop revolutionary technologies that enable the direct visualization and functional profiling of human regulatory regions at the resolution of individual chromatin templates in intact cells, and to leverage the extraordinary cell selectivity and information content of regulatory DNA to pioneer novel biological and translational applications. The Center will specifically (i) develop technology to simultaneously visualize and localize in highly multiplexed fashion, regulatory DNA regions on individual chromatin templates within the intact cell nucleus; (ii) develop technology for activity-based profiling of regulatory regions; (iii) pioneer structural, functional and integrative applications of photogenomics using super-resolution "nanoscopic" techniques; (iv) enable photogenomics through development of revolutionary instrumentation for high-throughput, high-speed super-resolution microscopy; and (v) lay the foundation for translation of photogenomic techniques to solving common problems in the modern clinical diagnostic laboratory by integrating with existing clinical workflows. This will ensure that photogenomics wil bring the power of genomic analysis to the understanding of cells within the context of their tissue environment. In keeping with the mission of the CEGS program of training and outreach, this Center proposal outlines a strong multi-disciplinary post- doctoral program that will create a new breed of genomics researcher with expertise in genomics as well as advanced imaging techniques and analysis. In addition, through innovative and interactive programs, the Center will expose non-genomics researchers and pre-doctoral students to this new field.

描述（由申请人提供）：光基因组学中心的使命是开发革命性的技术，使基因组的直接可视化和功能分析成为可能。该中心将专门 (i) 开发同时可视化和本地化的技术。完整细胞核内单个染色质模板上的高度多重时尚、调控 DNA 区域；(ii) 开发基于活性的调控区域分析技术；(iii) 开创光基因组学的结构、功能和整合应用超分辨率“纳米”技术；（iv）通过开发高通量、高速超分辨率显微镜的革命性仪器来实现光基因组学；（v）为光基因组技术解决现代常见问题奠定基础；通过与现有的临床工作流程相结合，这将确保光基因组学能够利用基因组分析的力量来了解细胞的组织环境，从而符合 CEGS 培训和计划的使命。外展，该中心提案概述了一个强大的多学科博士后计划，该计划将创建一个此外，该中心还将通过创新和互动项目，让非基因组学研究人员和博士生接触这一新领域。