Molecular and serological surveillance of SARS-CoV-2 in companion and food animals, USA

美国伴侣动物和食用动物中 SARS-CoV-2 的分子和血清学监测

• 批准号: 10828615
• 负责人: Chengming Wang
• 金额: $ 3.37万
• 依托单位: AUBURN UNIVERSITY AT AUBURN
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10828615
• 关键词: Molecular; serological; surveillance; SARS; CoV

Project Abstract/Summary Molecular and serological surveillance of SARS-CoV-2 in companion and food animals, USA Monitoring SARS-CoV-2 infections in animals is crucial for better understanding the virus and its potential for transmission between species. The virus has already been shown to infect a range of animals, including minks, dogs, and cats. By monitoring animal infections, potential reservoirs of the virus can be identified and new strains that may be more transmissible or virulent can be prevented. Additionally, monitoring animal infections ensures the safety of both animal and human populations by identifying and controlling infections in animals. In this study, we will perform PCR and ELISAs to detect SARS-CoV-2 virus and antibodies in nationwide collected dog samples (n=800), cat samples (n=800), and in horse (n=200) and cattle (n=200) samples from Alabama. SARS-CoV-2 Reverse- Transcription FRET-PCR will be performed to detect the virus, followed by DNA sequencing and whole-genome sequencing. The ID Screen® SARS-CoV-2 Double Antigen ELISA and SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) will be used to detect antibodies against SARS-CoV-2 infection, and positive and questionable samples will be sent to the United States Department of Agriculture (USDA) National Veterinary Services Laboratories (NVSL) for SARS-CoV-2 virus neutralization tests to confirm results. Monitoring animal populations for SARS-CoV-2 infections is essential to prevent the spread of the virus to humans and identify potential reservoirs of the virus. This study will also help to understand the epidemiology of SARS-CoV-2 in dogs, cats, and horses, as well as food animals in the USA.

项目摘要/总结 伴侣和食品中 SARS-CoV-2 的分子和血清学监测 动物, 美国 监测动物中的 SARS-CoV-2 感染对于更好地了解至关重要 该病毒及其在物种之间传播的可能性已经得到了证实。 通过监测动物，显示该病毒可感染一系列动物，包括水貂、狗和猫。 可以识别感染、潜在的病毒储存库以及可能出现的新毒株 此外，还可以通过监测动物感染来预防更具传染性或毒性的疾病。 通过识别和控制确保动物和人类的安全 动物感染。 在本研究中，我们将进行 PCR 和 ELISA 来检测 SARS-CoV-2 病毒并 全国收集的狗样本（n=800）、猫样本（n=800）和 来自阿拉巴马州的马 (n=200) 和牛 (n=200) 样本。 将进行转录 FRET-PCR 检测病毒，然后进行 DNA 检测 ID Screen® SARS-CoV-2 双测序和全基因组测序。 将使用抗原 ELISA 和 SARS-CoV-2 替代病毒中和试验 (sVNT) 检测抗 SARS-CoV-2 感染的抗体，以及阳性和可疑抗体 样品将被送往美国农业部（USDA）国家 兽医服务实验室 (NVSL) 进行 SARS-CoV-2 病毒中和测试，以 确认结果。 监测动物种群的 SARS-CoV-2 感染对于预防至关重要 这项研究旨在了解病毒向人类的传播并确定病毒的潜在储存者。 还将有助于了解 SARS-CoV-2 在狗、猫和马中的流行病学， 以及美国的食用动物。