Enhancing Performance of Antimicrobial Susceptibility Testing (AST) in Support of Vet-LIRN Activities

提高抗菌药物敏感性测试 (AST) 的性能以支持 Vet-LIRN 活动

• 批准号: 10828498
• 负责人: Kelli Maddock
• 金额: $ 8.46万
• 依托单位: NORTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10828498
• 关键词: Enhancing; Performance; Antimicrobial; Susceptibility; Testing

Project Summary/Abstract: This comparison study is intended to compare Sensititre™ Vet AST COMPGP1F/COMPGN1F plates to bioMérieux Vitek® AST-GP81/AST-GN96 cards for performance of antimicrobial susceptibility testing (AST) results. This method comparison will be performed on common bacterial companion animal pathogens such as Escherichia coli, Enterococcus species, Staphylococcus pseundintermedius, Pseudomonas aeruginosa, and other Enterobacterales isolates. These bacterial pathogens may be shared between companion animals and humans; therefore, antimicrobial resistance (AMR) of these isolates is a critical concern. AST results on companion animals are significant for prediction of treatment outcome and are valuable comparisons to AMR trends occurring in humans. Although widely used in veterinary microbiology, AST plates manufactured by the currently dominating vendor, Sensititre™, are lacking the necessary range of antimicrobials to adequately detect common resistance mechanisms which would allow for appropriate comparison to AMR trends occurring in humans. The bioMérieux Vitek is commonly used in human microbiology laboratories; AST cards designed for this system incorporate ranges of antimicrobial concentrations of clinical and epidemiological interest for key indicators of antimicrobial resistance. Although the manufacturer has products designed for veterinary use, few laboratories in the United States use the bioMérieux Vitek instrument for AST. To date, there are no published studies comparing Sensititre™ AST to bioMérieux Vitek. Findings of this methods comparison will be published, which will provide objective data that veterinary diagnostic laboratories can use to determine which method is most appropriate for their laboratories. This comparison study will enhance AMR surveillance capabilities of the NDSU VDL and will directly support Vet-LIRN initiatives of protecting public health. This study may identify a reliable and affordable second vendor for AST in veterinary microbiology, which also ensures that all vendors continue offering useful veterinary products and encourages routine improvements of products offered to veterinary diagnostic laboratories.

项目摘要/摘要： 该比较研究旨在比较Sensitre™Vet AST CompGP1F/Compgn1f BioMérieuxVitek®AST-GP81/AST-GN96卡的板块抗菌剂 敏感性测试（AST）结果。此方法比较将对共同进行 细菌伴侣的动物病原体，例如大肠杆菌，肠球菌， Pseundintermedius葡萄球菌，铜绿假单胞菌和其他肠杆菌 分离物。这些细菌病原体可以在伴侣动物和人类之间共享。 因此，这些分离株的抗菌耐药性（AMR）是一个关键问题。 AST结果 伴侣动物对于预测治疗结果很重要，很有价值 与人类发生的AMR趋势进行比较。虽然广泛用于兽医 微生物学是由当前主导的供应商Sensitre™制造的AST板 缺少必要的抗菌剂范围以充分检测到公共电阻 允许与AMR趋势进行适当比较的机制发生在人类中。 BioMérieuxVitek通常用于人类微生物实验室。 AST卡 专为该系统设计的抗菌浓度的临床和 抗菌素抵抗的关键指标的流行病学兴趣。虽然 制造商拥有用于兽医使用的产品，美国很少有实验室 AST的BioMérieuxVitek仪器。迄今为止，还没有比较的研究 Sensitre™AST至BioMérieuxVitek。这种方法比较的结果将发布， 它将提供兽医诊断实验室可以使用的客观数据来确定 哪种方法最适合其实验室。该比较研究将增强 NDSU VDL的AMR监视功能，并将直接支持兽医奖计划 保护公共卫生。这项研究可能会确定可靠且负担得起的第二供应商 兽医微生物学的AST，这也确保所有供应商都会继续提供有用 兽医产品并鼓励提供兽医产品的产品的常规改进 诊断实验室。