Division site determination in Gram-positive bacteria

革兰氏阳性菌分裂位点的测定

• 批准号: 10798857
• 负责人: Prahathees Jai Eswara
• 金额: $ 24.99万
• 依托单位: UNIVERSITY OF SOUTH FLORIDA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10798857
• 关键词: Address; Antibiotics; Bacillus subtilis; Bacteria; Bacterial Antibiotic Resistance; Biological Process; Cell division; Cells; Cytokinesis; Data; Drug Targeting; Escherichia coli; Gram-Positive Bacteria; Knowledge; Modeling; Nature; Organism; Process; Public Health; Publications; Reporting; Rod; STEM research; Shapes; Site; Staphylococcus aureus; System; Time; design; fighting; innovation; model organism; novel; stem cell division

Cell division is an essential biological process. Majority of the attempts to understand bacterial cell division stems from research performed in rod-shaped model organisms, Gram-negative Escherichia coli and Gram-positive Bacillus subtilis. Even in these model bacteria, new reports have exposed the presence of as-yet unidentified cell division factors. Unlike these rod-shaped organisms, which divide in the middle perpendicular to the long axis, spherical Staphylococcus aureus cells divide sequentially in orthogonal planes. Though this nature of S. aureus cell division has been known for 40 years, no viable models exist at this time to explain how this mode of cytokinesis is achieved and its significance in this organism. S. aureus also lacks a key cell division regulatory system (Min system) that is present in model organisms. Our proposed project is designed to address these major knowledge gaps in the field of bacterial cell division, specifically in the Gram-positive model bacterium B. subtilis and the spherical bacterium of significant

细胞分裂是一个重要的生物过程。大多数了解细菌的尝试 细胞分裂源于在革兰氏阴性杆状模型生物体中进行的研究 大肠杆菌和革兰氏阳性枯草芽孢杆菌。即使在这些模型细菌中，新的报告也表明 暴露了尚未识别的细胞分裂因子的存在。与这些杆状生物不同， 球形金黄色葡萄球菌细胞在垂直于长轴的中间分裂 在正交平面上依次进行。尽管金黄色葡萄球菌细胞分裂的这种性质已为人所知 40 多年来，目前还没有可行的模型来解释这种胞质分裂模式是如何实现的及其 在这个有机体中的重要性。金黄色葡萄球菌还缺乏关键的细胞分裂调控系统（Min系统） 存在于模式生物中。我们提出的项目旨在解决这些主要问题 细菌细胞分裂领域的知识差距，特别是革兰氏阳性模型细菌 B.枯草芽孢杆菌和显着的球形细菌